The timing of established detrusor hyperreflexia in a rat model of neuropathic bladder.

Abstract

BACKGROUND:

Proper timing of catheter insertion and the use of a suitable surgical method are essential parts of producing rat models to evaluate neuropathic bladder following spinal cord injury (SCI).

METHODS:

Thirty-two female Sprague-Dawley rats were randomly allocated into four groups. Group 1 underwent surgical laminectomy using the classic method. Group 2 underwent SCI 7 d following insertion of the catheter, and group 3 underwent sham operation. For bladder catheterization, a 4.5 Fr catheter was fixed into the bladder and tunneled beneath the skin to reach out at the nape of the neck. Group 4 underwent urodynamic study via bladder catheter prior to surgery and every 10 d following the operation to determine the exact time of establishing neuropathic bladder following spinal shock. The animals' survival rate and bladder wall's histopathologic changes were assessed 30 d following the operation.

RESULTS:

Simultaneous suprapubic catheter placement raised the mortality rate in group 1 in comparison with group 2. Repeated urodynamic study in group 4 showed hypertonic behavior in the bladder 10 d after SCI, with significantly increased leak point pressure and bladder capacity; however, the end filling pressure and constant neuropathic bladder on cystometric indices are attained from 20 d after the operation.

CONCLUSIONS:

Insertion of a bladder catheter 1 wk prior to SCI provides an applicable route for repeated cystometric studies in rats. The results demonstrate that sustained bladder overactivity is established in rats 20 d after SCI and animals are ready for further experiments on neuropathic bladder dysfunction following this period.

• Authors: Abdol-Mohammad Kajbafzadeh, Payam Mohammadinejad, Asal Hojjat, Behtash Ghazi Nezami, Saman Shafat Talab, Hamed Emami, Shadi Abdar Esfahani
• Journal: The Journal of surgical research (J Surg Res) Vol. 178 Issue 1 Pg. 346-51 (Nov 2012) ISSN: 1095-8673 [Electronic] United States
• PMID: 22883436 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Topics

• Animals
• Cystostomy (methods)
• Disease Models, Animal
• Female
• Laminectomy (methods)
• Postoperative Complications (mortality, physiopathology)
• Rats
• Rats, Sprague-Dawley
• Reproducibility of Results
• Spinal Cord Injuries (mortality, physiopathology)
• Time Factors
• Urinary Bladder, Neurogenic (mortality, physiopathology)
• Urinary Bladder, Overactive (mortality, physiopathology)
• Urinary Catheterization (methods)