Highly emissive PEG-encapsulated conjugated polymer nanoparticles.

Abstract

A novel bioimaging probe based on a conjugated polymer, poly(9,9-dihexylfluorene-alt-2,1,3-benzoxadiazole) (PFBD), is demonstrated. Transfer of the hydrophobic polymer into water using a short chain poly(ethylene glycol) (PEG) resulted in conjugated polymer nanoparticles (PEG-PFBD) with a fluorescence quantum yield of 46%. The PEG-PFBD nanoparticles possessed several desirable structural and photophysical properties, such as colloidal stability in a broad range of pH values, sub-20 nm particle size, the presence of surface chemical functionality, as well as desirable excitation and emission spectra, for bioimaging applications. PEG-PFBD nanoparticles were conjugated with cyclic RGDfK targeting peptide for labeling of membrane α(V)β(3) integrin receptors on live HT-29 adenocarcinoma cells. Single nanoparticle microscopy revealed that the PEG-capped PFBD nanoparticles exhibit at least ten times higher emitted photon counts than single quantum dots (QD655) of comparable size. In addition, Fluorescence Lifetime Imaging Microscopy (FLIM) of single PEG-PFBD nanoparticles revealed that the nanoparticles display a clearly resolvable single nanoparticle fluorescence lifetime.

• Authors: Yuqiong Li, Jie Liu, Bin Liu, Nikodem Tomczak
• Journal: Nanoscale (Nanoscale) Vol. 4 Issue 18 Pg. 5694-702 (Sep 21 2012) ISSN: 2040-3372 [Electronic] England
• PMID: 22878417 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)