Quantitative proton nuclear magnetic resonance evaluation and total assignment of the capsular polysaccharide Neisseria meningitidis serogroup X.

Abstract

Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, Y, and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional (13)C NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving (13)C, (1)H, and (31)P nuclei.

• Authors: Raine Garrido, Alain Puyada, Anaís Fernández, Majela González, Ubel Ramírez, Felix Cardoso, Yury Valdés, Domingo González, Violeta Fernández, Vicente Vérez, Hermán Vélez
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 70 Pg. 295-300 (Nov 2012) ISSN: 1873-264X [Electronic] England
• PMID: 22877873 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Carbon Isotopes
• Phosphorus Isotopes
• Polysaccharides, Bacterial
• Protons

Topics

• Bacterial Capsules (chemistry, immunology)
• Carbohydrate Conformation
• Carbon Isotopes
• Linear Models
• Molecular Structure
• Neisseria meningitidis (chemistry, immunology)
• Nuclear Magnetic Resonance, Biomolecular (methods)
• Phosphorus Isotopes
• Polysaccharides, Bacterial (chemistry, immunology)
• Protons
• Reproducibility of Results