Abstract | PURPOSE: METHODS: Twenty-four Sprague-Dawley rats were used in this study. We induced I/R injury by clamping the optic nerve for 30 minutes and then releasing it. The vitreous bodies were obtained from the experimental and control subjects 24, 48, and 72 hours after I/R injury. Two-dimensional electrophoresis was performed, and the targeted spots were further investigated using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, spectrophotometry, Western blotting, and histological examination. RESULTS: After I/R injury, 23 spots were identified as crystallins. The βB2 crystallins were transcriptionally and post-translationally regulated, whereas the αB crystallins were controlled by post-translational modifications in the vitreous bodies of the rats. The total amounts of αA and β crystallins (including isotypes of β crystalline) had increased 48 hours after injury. The phosphorylation of αB crystallin (at serine residues 19, 45, and 59) was significantly increased 48 hours later, whereas phosphorylation of ERK1/2 showed the greatest decrease. CONCLUSIONS: During hypoxic and oxidation stress, our results suggest that phosphorylated αB crystalline inhibits RAS, resulting in the inactivation of ERK1/2. The phosphorylation of αB crystallin may be associated with the inflammatory suppression in the vitreous body via the I/R injury model system.
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Authors | Seong Min Hong, Yun Sik Yang |
Journal | Korean journal of ophthalmology : KJO
(Korean J Ophthalmol)
Vol. 26
Issue 4
Pg. 248-54
(Aug 2012)
ISSN: 2092-9382 [Electronic] Korea (South) |
PMID | 22870022
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Blotting, Western
- Oxidative Stress
- Phosphorylation
- Protein Processing, Post-Translational
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(metabolism, pathology)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Vitreous Body
(metabolism, pathology)
- beta-Crystallins
(metabolism)
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