Abstract | OBJECTIVES: METHODS: Total RNA was extracted from 16 tissue specimens. Expression analysis was carried out using Agilent 4 × 44 k arrays. Gene ontology analysis was used to define pathways altered in bronchioloalveolar progression. Differentially expressed candidate genes were validated using quantitative real-time PCR. The statistical analysis was carried out according to the methods of the paired t-test. RESULTS: CONCLUSIONS: Microarray-based expression profiling revealed interesting novel candidate genes in BAC and adenocarcinoma with bronchioloalveolar features. The MAPK pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with the pure BAC pathway, which is worthy of being explored because it could partially explain the mechanism of the progression of BAC to adenocarcinoma with bronchioloalveolar features.
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Authors | Wen-Cheng Zhang, Zhen-Fa Zhang, Jian You, Chang-Li Wang |
Journal | European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
(Eur J Cardiothorac Surg)
Vol. 43
Issue 4
Pg. 765-71
(Apr 2013)
ISSN: 1873-734X [Electronic] Germany |
PMID | 22864788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neoplasm Proteins
- RNA, Messenger
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Topics |
- Adenocarcinoma
(chemistry, classification, diagnosis, metabolism)
- Adenocarcinoma of Lung
- Adenocarcinoma, Bronchiolo-Alveolar
(classification, diagnosis, genetics, metabolism)
- Aged
- Aged, 80 and over
- Diagnosis, Differential
- Female
- Gene Expression Profiling
- Humans
- Lung Neoplasms
(chemistry, classification, diagnosis, genetics, metabolism)
- Male
- Middle Aged
- Neoplasm Proteins
(biosynthesis, genetics)
- Neoplasm Staging
- Oligonucleotide Array Sequence Analysis
- RNA, Messenger
(analysis, metabolism)
- Real-Time Polymerase Chain Reaction
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