Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Fasting plasma and salivary endocannabinoids and N-acylethanolamines were measured through liquid mass spectrometry in 12 normal weight and 12 obese, insulin-resistant subjects. Salivary endocannabinoids and N-acylethanolamines were evaluated in the same cohort before and after the consumption of a meal. Changes in salivary endocannabinoids and N-acylethanolamines after body weight loss were investigated in a second group of 12 obese subjects following a 12-weeks lifestyle intervention program. The levels of mRNAs coding for enzymes regulating the metabolism of endocannabinoids, N-acylethanolamines and of cannabinoid type 1 (CB(1)) receptor, alongside endocannabinoids and N-acylethanolamines content, were assessed in human salivary glands. The endocannabinoids 2-arachidonoylglycerol (2-AG), N-arachidonoylethanolamide ( anandamide, AEA), and the N-acylethanolamines ( oleoylethanolamide, OEA and palmitoylethanolamide, PEA) were quantifiable in saliva and their levels were significantly higher in obese than in normal weight subjects. Fasting salivary AEA and OEA directly correlated with BMI, waist circumference and fasting insulin. Salivary endocannabinoids and N-acylethanolamines did not change in response to a meal. CB(1) receptors, ligands and enzymes were expressed in the salivary glands. Finally, a body weight loss of 5.3% obtained after a 12-weeks lifestyle program significantly decreased salivary AEA levels. CONCLUSIONS/SIGNIFICANCE:
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Authors | Isabelle Matias, Blandine Gatta-Cherifi, Antoine Tabarin, Samantha Clark, Thierry Leste-Lasserre, Giovanni Marsicano, Pier Vincenzo Piazza, Daniela Cota |
Journal | PloS one
(PLoS One)
Vol. 7
Issue 7
Pg. e42399
( 2012)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22860123
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- DNA Primers
- Endocannabinoids
- RNA, Messenger
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Topics |
- Adult
- Base Sequence
- Biomarkers
(metabolism)
- Case-Control Studies
- Cohort Studies
- DNA Primers
- Endocannabinoids
(metabolism)
- Female
- Humans
- Male
- Mass Spectrometry
- Middle Aged
- Obesity
(metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(genetics)
- Saliva
(metabolism)
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