Dehydration from conserved stem regions is fundamental for ligand-dependent conformational transition of the adenine-specific riboswitch.

Abstract

Conformational dynamics and observed equilibrium constants for ligand binding of the adenine-specific riboswitch (add-A riboswitch) in the absence of Mg(2+) and presence of various concentrations of poly-ethylene glycol having an average molecular weight of 200 indicated that 54.2 water molecules were released from P2 and P3 stem regions of the add-A riboswitch during conformational transition upon the binding of 2-aminopurine, an analog of the natural ligand adenine.

• Authors: Vinit Kumar, Tamaki Endoh, Kentaro Murakami, Naoki Sugimoto
• Journal: Chemical communications (Cambridge, England) (Chem Commun (Camb)) Vol. 48 Issue 78 Pg. 9693-5 (Oct 09 2012) ISSN: 1364-548X [Electronic] England
• PMID: 22854864 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ligands
• Riboswitch
• Water
• Polyethylene Glycols
• Adenine

Topics

• Adenine (chemistry)
• Desiccation
• Ligands
• Molecular Weight
• Nucleic Acid Conformation
• Polyethylene Glycols (chemistry)
• Riboswitch
• Water (chemistry)