Abstract | BACKGROUND: METHODS: Four NSCLC cell lines were treated with extracellular S100A4, and ephrin-A1 and osteopontin expression was analyzed by real time RT-PCR and Western blotting. Immunohistochemical staining for S100A4, ephrin-A1 and osteopontin was performed on tissue microarrays containing primary tumor samples from a cohort of 217 prospectively recruited NSCLC patients, and associations with clinicopathological parameters were investigated. RESULTS: CONCLUSIONS: Our findings suggest that S100A4, ephrin-A1 and osteopontin are involved in the biology of NSCLC, and further investigation of their potential use as biomarkers in NSCLC is warranted.
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Authors | Ane Kongsgaard Rud, Marius Lund-Iversen, Gisle Berge, Odd Terje Brustugun, Steinar K Solberg, Gunhild M Mælandsmo, Kjetil Boye |
Journal | BMC cancer
(BMC Cancer)
Vol. 12
Pg. 333
(Aug 01 2012)
ISSN: 1471-2407 [Electronic] England |
PMID | 22853000
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Ephrin-A1
- RNA, Messenger
- S100 Calcium-Binding Protein A4
- S100 Proteins
- Osteopontin
- S100A4 protein, human
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Topics |
- Adult
- Aged
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Carcinoma, Non-Small-Cell Lung
(metabolism)
- Cell Line, Tumor
- Ephrin-A1
(metabolism)
- Female
- Humans
- Immunohistochemistry
- Lung Neoplasms
(metabolism)
- Male
- Microarray Analysis
- Middle Aged
- Osteopontin
(metabolism)
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- S100 Calcium-Binding Protein A4
- S100 Proteins
(metabolism)
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