Abstract |
Chronic bee paralysis virus (CBPV) infection causes chronic paralysis and loss of workers in honey bee colonies around the world. Although CBPV shows a worldwide distribution, it had not been molecularly detected in Japan. Our investigation of Apis mellifera and Apis cerana japonica colonies with RT-PCR has revealed CBPV infection in A. mellifera but not A. c. japonica colonies in Japan. The prevalence of CBPV is low compared with that of other viruses: deformed wing virus (DWV), black queen cell virus (BQCV), Israel acute paralysis virus (IAPV), and sac brood virus (SBV), previously reported in Japan. Because of its low prevalence (5.6%) in A. mellifera colonies, the incidence of colony losses by CBPV infection must be sporadic in Japan. The presence of the (-) strand RNA in dying workers suggests that CBPV infection and replication may contribute to their symptoms. Phylogenetic analysis demonstrates a geographic separation of Japanese isolates from European, Uruguayan, and mainland US isolates. The lack of major exchange of honey bees between Europe/mainland US and Japan for the recent 26 years (1985-2010) may have resulted in the geographic separation of Japanese CBPV isolates.
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Authors | Tomomi Morimoto, Yuriko Kojima, Mikio Yoshiyama, Kiyoshi Kimura, Bu Yang, Tatsuhiko Kadowaki |
Journal | Viruses
(Viruses)
Vol. 4
Issue 7
Pg. 1093-103
(07 2012)
ISSN: 1999-4915 [Electronic] Switzerland |
PMID | 22852042
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Bees
(virology)
- Female
- Insect Viruses
(classification, genetics, isolation & purification)
- Japan
- Molecular Sequence Data
- Phylogeny
- RNA Viruses
(classification, genetics, isolation & purification)
- Viral Proteins
(genetics)
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