Abstract | BACKGROUND AND AIM: METHODS: Cirrhotic rats were created by the administration of carbon tetrachloride (CCl(4) ) twice weekly for 10 weeks. From week 7, rat bone marrow-derived EPCs were injected via the tail vein in this model once a week for 4 weeks. Endothelial NOS (eNOS), vascular endothelial growth factor ( VEGF) and caveolin expressions were examined by Western blots. Hepatic tissue ET-1 was measured by a radioimmunoassay (RIA). Portal venous pressure, mean aortic pressure, and hepatic blood flow were measured. RESULTS: Endothelial progenitor cell transplantation reduced liver fibrosis, α-smooth muscle actin-positive cells, caveolin expression, ET-1 concentration and portal venous pressure. EPC transplantation increased hepatic blood flow, protein levels of eNOS and VEGF. Immunohistochemical analyses of eNOS and isolectin B4 demonstrated that the livers of EPC-transplanted animals had markedly increased vascular density, suggesting reconstitution of sinusoidal blood vessels with endothelium. CONCLUSIONS:
|
Authors | Masaharu Sakamoto, Toru Nakamura, Takuji Torimura, Hideki Iwamoto, Hiroshi Masuda, Hironori Koga, Mitsuhiko Abe, Osamu Hashimoto, Takato Ueno, Michio Sata |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 28
Issue 1
Pg. 168-78
(Jan 2013)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 22849788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2012 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd. |
Chemical References |
- Caveolins
- Endothelin-1
- Vascular Endothelial Growth Factor A
- Carbon Tetrachloride
- Nitric Oxide Synthase Type III
|
Topics |
- Animals
- Blood Vessels
(physiopathology)
- Carbon Tetrachloride
- Caveolins
(metabolism)
- Cell Proliferation
- Endothelial Cells
(physiology, transplantation)
- Endothelin-1
(metabolism)
- Endothelium
(enzymology, metabolism)
- Hypertension, Portal
(etiology, physiopathology, therapy)
- Liver Circulation
- Liver Cirrhosis, Experimental
(chemically induced, complications, physiopathology)
- Male
- Nitric Oxide Synthase Type III
(metabolism)
- Portal Pressure
- Rats
- Rats, Wistar
- Stem Cell Transplantation
- Vascular Endothelial Growth Factor A
(metabolism)
|