Abstract | BACKGROUND: METHODS: On day 1, Sprague-Dawley rats were intratracheally instilled with 25 U kg⁻¹ pancreatic porcine elastase or saline. On day 7, they received an intratracheal instillation of TiO₂ or CB (at 100 and 500 μg) dispersed in bovine serum albumin or bovine serum albumin alone. Animals were sacrificed at days 8 or 21, and bronchoalveolar lavage (BAL) cellularity, histological analysis of inflammation and emphysema, and lung mRNA expression of heme oxygenase-1 (HO-1), interleukin-1β (IL-1β), macrophage inflammatory protein-2, monocyte chemotactic protein-1, and matrix metalloprotease (MMP)-1, and -12 were measured. In addition, pulmonary MMP-12 expression was also analyzed at the protein level by immunohistochemistry. RESULTS: TiO₂ NPs per se did not modify the parameters investigated, but CB NPs increased perivascular/peribronchial infiltration, and macrophage MMP-12 expression, without inducing emphysema. Elastase administration increased BAL cellularity, histological inflammation, HO-1, IL-1β and macrophage MMP-12 expression and induced emphysema. Exposure to TiO₂ NPs did not modify pulmonary responses to elastase, but exposure to CB NPs aggravated elastase-induced histological inflammation without aggravating emphysema. CONCLUSIONS:
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Authors | Agnès Roulet, Lucie Armand, Maylis Dagouassat, Françoise Rogerieux, Angélique Simon-Deckers, Esther Belade, Jeanne Tran Van Nhieu, Sophie Lanone, Jean-Claude Pairon, Ghislaine Lacroix, Jorge Boczkowski |
Journal | BMC pulmonary medicine
(BMC Pulm Med)
Vol. 12
Pg. 38
(Jul 31 2012)
ISSN: 1471-2466 [Electronic] England |
PMID | 22849372
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1beta
- Soot
- titanium dioxide
- Titanium
- Heme Oxygenase-1
- Pancreatic Elastase
- Matrix Metalloproteinase 12
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Topics |
- Animals
- Bronchoalveolar Lavage
- Heme Oxygenase-1
(metabolism)
- Interleukin-1beta
(metabolism)
- Intubation, Intratracheal
- Male
- Matrix Metalloproteinase 12
(metabolism)
- Models, Animal
- Nanoparticles
(administration & dosage, adverse effects)
- Pancreatic Elastase
(adverse effects)
- Pneumonia
(chemically induced, metabolism, pathology)
- Pulmonary Emphysema
(chemically induced, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
- Soot
(administration & dosage, adverse effects)
- Titanium
(administration & dosage, adverse effects)
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