Differential effects of high-fat-diet rich in lard oil or soybean oil on osteopontin expression and inflammation of adipose tissue in diet-induced obese rats.

Abstract

PURPOSE:

To examine the effect of different dietary fat types on osteopontin (OPN) expressions and inflammation of adipose tissues in diet-induced obese rats.

METHODS:

Male Sprague-Dawley rats were randomly assigned to one control group fed standard diet (LF, n = 10) and two high-fat diet groups fed isoenergy diet rich in lard or soybean oil (HL or HS, n = 45 each). Diet-induced obese rats in HL and HS group were then subdivided into two groups either continuously fed high-fat diet or switched to low-fat diet for 8 more weeks. Fasting serum glucose, insulin, and OPN concentrations were assayed and QUICKI was calculated; the expression of OPN, IL-6, IL-10, TNF-α, NF-κB, and F4/80 in adipose tissue was determined.

RESULTS:

Both high-fat diets lead to comparable development of obesity characterized by insulin resistance and adipose tissue inflammation. Obese rats continuously fed high-fat diet rich in lard oil exhibited the highest fasting serum insulin level and adipose tissue OPN, F4/80, TNF-α, and NF-κB expression level. In both high-fat diet groups, switching to low-fat diet resulted in less intra-abdominal fat mass, decreased expression of F4/80, TNF-α, and NF-κB, while decreased OPN expression was only observed in lard oil fed rats after switching to low-fat diet.

CONCLUSIONS:

Reducing diet fat or replacing lard oil with soybean oil in high-fat diet alleviates obesity-related inflammation and insulin resistance by attenuating the upregulation of OPN and macrophage infiltration into adipose tissue induced by high-fat diet.

• Authors: Xiaoke Wang, Mengjie Cheng, Min Zhao, Aiguo Ge, Fangfang Guo, Min Zhang, Yanhong Yang, Liegang Liu, Nianhong Yang
• Journal: European journal of nutrition (Eur J Nutr) Vol. 52 Issue 3 Pg. 1181-9 (Apr 2013) ISSN: 1436-6215 [Electronic] Germany
• PMID: 22847642 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Dietary Fats
• Spp1 protein, rat
• Osteopontin
• Soybean Oil
• lard

Topics

• Adipose Tissue, White (immunology, metabolism, pathology)
• Adiposity
• Animals
• Cytokines (blood, genetics, metabolism)
• Diet, Fat-Restricted
• Diet, High-Fat (adverse effects)
• Dietary Fats (administration & dosage, adverse effects)
• Down-Regulation
• Hyperinsulinism (etiology, prevention & control)
• Insulin Resistance
• Intra-Abdominal Fat (immunology, metabolism, pathology)
• Macrophage Activation
• Male
• Obesity (diet therapy, immunology, metabolism, pathology)
• Osteopontin (blood, genetics, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Soybean Oil (administration & dosage, adverse effects, therapeutic use)
• Specific Pathogen-Free Organisms