Prevalence of hepatitis B virus in chronic hepatitis B patients.

Abstract

The aim of the current study was to detect HBV by Real time - PCR in chronic hepatitis B patients. Fifty-eight sera of chronic hepatitis B patients were subjected during the period March 2009 to April 2010 in Ilam cities in West of Iran. Sera assayed by real-time PCR and ELISA methods. Twenty serum samples from healthy volunteers and non-hepatitis B patients and negative for hepatitis B seromarkers served as negative controls for the study. Among fifty-eight sera, ELISA showed fifty-five (94.8%) of the samples were positive for HBsAg and three (5.2%) negative results obtained while real-time PCR specified fifty-eight (100%) positive results in chronic hepatitis B patients. HBsAg status did not necessarily reflect HBV DNA level in the serum, as 5.2% of chronic Hepatitis B patients were positive for HBV DNA but negative for HBsAg. HBV DNA was not found to be positive amongst any of the negative controls. Real time - PCR is a sensitive and reproducible assay for HBV DNA quantization.

• Authors: Nourkhoda Sadeghifard, Reza Mohebi, Zamberi Sekawi, Sobhan Ghafourian, Hossien Kazemian, Mitra Rezaee
• Journal: Roumanian archives of microbiology and immunology (Roum Arch Microbiol Immunol) 2012 Jan-Mar Vol. 71 Issue 1 Pg. 53-5 ISSN: 1222-3891 [Print] Romania
• PMID: 22838221 (Publication Type: Journal Article)

Chemical References

• DNA, Viral
• Hepatitis B Surface Antigens

Topics

• DNA, Viral (analysis)
• Hepatitis B Surface Antigens (analysis, genetics)
• Hepatitis B virus (genetics, isolation & purification)
• Hepatitis B, Chronic (virology)
• Humans
• Prevalence