Paraoxonase 1 (PON1) 55 polymorphism, lipid profiles and psoriasis.

Abstract

BACKGROUND:

Paraoxonase 1 (PON1) is a serum high-density lipoprotein-bound enzyme with antioxidant function. It hydrolyses lipid peroxides, protecting low-density lipoproteins from oxidative modifications. Patients with psoriasis are at greater risk of oxidative stress, which is associated with abnormal plasma lipid metabolism.

OBJECTIVES:

In this study, association of the PON1 55 M allele with serum arylesterase (ARE) activity, malondialdehyde (MDA), lipid profiles and psoriasis was investigated.

METHODS:

The present case-control study consisted of 100 patients with psoriasis with and without cardiovascular diseases (mean age 35·3 years) and 100 sex- and age-matched unrelated healthy controls (mean age 35·7 years) from the population of western Iran. The PON1 55 Met>Leu polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. Serum ARE activity, MDA, and lipid and apolipoprotein levels were determined spectrophotometrically, by high-performance liquid chromatography and by enzyme assay, respectively.

RESULTS:

The presence of the PON1 55 M allele was found to be associated with psoriasis (odds ratio = 1·96, P = 0·017). The patients with psoriasis with the PON1 M (M/L + M/M) allele had higher MDA levels (4·12 ± 0·88 vs. 2·24 ± 0·55 μmol L(-1) , P < 0·001), apolipoprotein B (APOB)/APOA1 ratio (0·91 ± 0·66 vs. 0·66 ± 0·35, P = 0·004), APOB (111 ± 38·7 vs. 88·3 ± 22·5 mg mL(-1) , P = 0·001) and lipoprotein(a) [LP(a)] (21·9 ± 18·4 vs. 15·8 ± 16·6 mg mL(-1) , P = 0·034), but lower ARE activity (39·6 ± 11 vs. 45·9 ± 11·8 U mL(-1) , P = 0·031) than the control subjects. ARE activity showed a significant positive correlation with APOA1 and a negative correlation with MDA concentration in patients with psoriasis.

CONCLUSIONS:

The PON1 55 M allele is a risk factor for psoriasis. Carriers of this allele have high levels of MDA, APOB and LP(a), a high APOB/APOA1 ratio and low ARE activity. These results indicate that oxidative stress, impairment of the antioxidant system and abnormal lipid metabolism may play a role in the pathogenesis and progression of psoriasis and its related complications. These data suggest that patients with psoriasis are more susceptible to vascular diseases.

• Authors: M Asefi, A Vaisi-Raygani, F Bahrehmand, A Kiani, Z Rahimi, H Nomani, A Ebrahimi, H Tavilani, T Pourmotabbed
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 167 Issue 6 Pg. 1279-86 (Dec 2012) ISSN: 1365-2133 [Electronic] England
• PMID: 22835076 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 The Authors. BJD © 2012 British Association of Dermatologists.

Chemical References

• Biomarkers
• Cholesterol, HDL
• Cholesterol, LDL
• Triglycerides
• Malondialdehyde
• Carboxylic Ester Hydrolases
• arylesterase
• Aryldialkylphosphatase
• PON1 protein, human

Topics

• Adolescent
• Adult
• Aged
• Alleles
• Aryldialkylphosphatase (genetics, metabolism)
• Biomarkers (metabolism)
• Carboxylic Ester Hydrolases (blood)
• Case-Control Studies
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Female
• Humans
• Lipid Peroxidation (physiology)
• Male
• Malondialdehyde (blood)
• Middle Aged
• Oxidative Stress (physiology)
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Psoriasis (blood, genetics)
• Risk Factors
• Triglycerides (blood)
• Young Adult