Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model.
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| Abstract |
Proneurotensin/neuromedin N (proNT/NMN), the precursor of neurotensin (NT) and neuromedin N (NMN), is produced by cancer tissues derived from the pancreas and colon. NT stimulates tumor growth and proliferation through its receptors; however, little is known about the precursor molecule in cancer tissues. We previously demonstrated that proNT/NMN is secreted from small cell lung carcinoma (SCLC) cell lines in serum-free conditioned medium, but not from non-small cell lung carcinoma (NSCLC) cell lines. It was suggested that this precursor may serve as a tumor marker for SCLC. In this study, we established in vivo xenograft models to evaluate the possibility of proNT/NMN as a specific tumor marker. SBC3 cells, derived from human SCLC, were inoculated into mice, and the proNT/NMN levels in plasma and tumor tissues were detected using specific antibodies. In contrast to control mouse plasma, the proNT/NMN levels in tumor-bearing mice increased as the tumors grew, and the elevated plasma proNT/NMN levels were decreased by tumor resection. Moreover, proNT/NMN was expressed in SBC3 tumors, suggesting that proNT/NMN was secreted into blood from the tumor, and this secretion may be specific to SCLC.
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| Authors | Kanako Wakabayashi-Nakao, Koji Maruyama, Hidee Ishii, Koji Muramatsu, Keiichi Hatakeyama, Keiichi Ohshima, Shun-Ichiro Ogura, Takashi Nakajima, Ken Yamaguchi, Tohru Mochizuki |
| Journal | Oncology reports (Oncol Rep) Vol. 28 Issue 4 Pg. 1181-6 (Oct 2012) ISSN: 1791-2431 [Electronic] Greece |
| PMID | 22825476 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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