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Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl₄-induced fibrotic liver.

AbstractBACKGROUND/AIM:
We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis.
PATIENTS AND METHODS:
BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data.
RESULTS:
GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining.
CONCLUSION:
Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis.
AuthorsGibran Ali, Muhammad S Masoud
JournalSaudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association (Saudi J Gastroenterol) 2012 Jul-Aug Vol. 18 Issue 4 Pg. 263-7 ISSN: 1998-4049 [Electronic] India
PMID22824770 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Actins
  • Albumins
  • Carbon Tetrachloride
  • Matrix Metalloproteinases
Topics
  • Actins (metabolism)
  • Albumins (metabolism)
  • Animals
  • Bone Marrow Cells (metabolism)
  • Bone Marrow Transplantation
  • Carbon Tetrachloride
  • Female
  • Liver Cirrhosis (chemically induced, metabolism, therapy)
  • Matrix Metalloproteinases (metabolism)
  • Mice
  • Mice, Inbred C57BL
  • Time Factors

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