HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Gallic acid-grafted chitooligosaccharides suppress antigen-induced allergic reactions in RBL-2H3 mast cells.

Abstract
In this study, a bioactive derivative of chitooligosaccharides (3-5 kDa) was synthesized via grafting of gallic acid onto chitooligosaccharides (G-COS) to enhance anti-allergic activity. Hence, G-COS was evaluated for its capabilities against allergic reactions in RBL-2H3 mast cells sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was revealed that G-COS exhibited significant inhibition on histamine release and production as well as intracellular Ca(2+) elevation at the concentration of 200μg/ml. Likewise, the suppressive effects of G-COS on expression and production of interleukin (IL)-4 and tumor necrosis factor (TNF)-α were evidenced. Moreover, G-COS treatment caused a remarkable blockade on degradation of inhibitory κB-α (IκB-α) protein, translocation of nuclear factor (NF)-κB, and phosphorylation of mitogen-activated protein kinases (MAPKs). Notably, the inhibitory activities of G-COS on allergic reactions were found as a consequence of suppression of FcεRI expression in antigen-stimulated cells. Accordingly, G-COS was suggested to be a promising candidate of novel inhibitors against allergic reactions.
AuthorsThanh-Sang Vo, Dai-Hung Ngo, Se-Kwon Kim
JournalEuropean journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (Eur J Pharm Sci) Vol. 47 Issue 2 Pg. 527-33 (Sep 29 2012) ISSN: 1879-0720 [Electronic] Netherlands
PMID22820028 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Anti-Allergic Agents
  • Antigens
  • Cytokines
  • Dinitrophenols
  • FCER1A protein, rat
  • NF-kappa B
  • Oligosaccharides
  • Receptors, IgE
  • dinitrophenyl-bovine serum albumin
  • Serum Albumin, Bovine
  • Immunoglobulin E
  • Gallic Acid
  • Mitogen-Activated Protein Kinases
  • Calcium
Topics
  • Animals
  • Anti-Allergic Agents (pharmacology)
  • Antigens (immunology)
  • Calcium (metabolism)
  • Cell Line
  • Cell Survival (drug effects)
  • Cytokines (immunology)
  • Dinitrophenols (immunology)
  • Gallic Acid (analogs & derivatives, pharmacology)
  • Histamine Release (drug effects)
  • Immunoglobulin E (immunology)
  • Mast Cells (drug effects, immunology)
  • Mitogen-Activated Protein Kinases (immunology)
  • NF-kappa B (immunology)
  • Oligosaccharides (pharmacology)
  • Rats
  • Receptors, IgE (immunology)
  • Serum Albumin, Bovine (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: