Abstract |
In this study, a bioactive derivative of chitooligosaccharides (3-5 kDa) was synthesized via grafting of gallic acid onto chitooligosaccharides (G-COS) to enhance anti-allergic activity. Hence, G-COS was evaluated for its capabilities against allergic reactions in RBL-2H3 mast cells sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was revealed that G-COS exhibited significant inhibition on histamine release and production as well as intracellular Ca(2+) elevation at the concentration of 200μg/ml. Likewise, the suppressive effects of G-COS on expression and production of interleukin (IL)-4 and tumor necrosis factor (TNF)-α were evidenced. Moreover, G-COS treatment caused a remarkable blockade on degradation of inhibitory κB-α (IκB-α) protein, translocation of nuclear factor (NF)-κB, and phosphorylation of mitogen-activated protein kinases (MAPKs). Notably, the inhibitory activities of G-COS on allergic reactions were found as a consequence of suppression of FcεRI expression in antigen-stimulated cells. Accordingly, G-COS was suggested to be a promising candidate of novel inhibitors against allergic reactions.
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Authors | Thanh-Sang Vo, Dai-Hung Ngo, Se-Kwon Kim |
Journal | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
(Eur J Pharm Sci)
Vol. 47
Issue 2
Pg. 527-33
(Sep 29 2012)
ISSN: 1879-0720 [Electronic] Netherlands |
PMID | 22820028
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-Allergic Agents
- Antigens
- Cytokines
- Dinitrophenols
- FCER1A protein, rat
- NF-kappa B
- Oligosaccharides
- Receptors, IgE
- dinitrophenyl-bovine serum albumin
- Serum Albumin, Bovine
- Immunoglobulin E
- Gallic Acid
- Mitogen-Activated Protein Kinases
- Calcium
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Topics |
- Animals
- Anti-Allergic Agents
(pharmacology)
- Antigens
(immunology)
- Calcium
(metabolism)
- Cell Line
- Cell Survival
(drug effects)
- Cytokines
(immunology)
- Dinitrophenols
(immunology)
- Gallic Acid
(analogs & derivatives, pharmacology)
- Histamine Release
(drug effects)
- Immunoglobulin E
(immunology)
- Mast Cells
(drug effects, immunology)
- Mitogen-Activated Protein Kinases
(immunology)
- NF-kappa B
(immunology)
- Oligosaccharides
(pharmacology)
- Rats
- Receptors, IgE
(immunology)
- Serum Albumin, Bovine
(immunology)
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