Abstract |
Adenylate kinases (AK) play a key role in nucleotide signaling processes and energy metabolism by catalyzing the reversible conversion of ATP and AMP to 2 ADP. In the malaria parasite Plasmodium falciparum this reaction is mediated by AK1, AK2, and a GTP:AMP phosphotransferase (GAK). Here, we describe two additional adenylate kinase-like proteins: PfAKLP1, which is homologous to human AK6, and PfAKLP2. Using GFP-fusion proteins and life cell imaging, we demonstrate a cytosolic localization for PfAK1, PfAKLP1, and PfAKLP2, whereas PfGAK is located in the mitochondrion. PfAK2 is located at the parasitophorous vacuole membrane, and this localization is driven by N-myristoylation.
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Authors | Jipeng Ma, Stefan Rahlfs, Esther Jortzik, R Heiner Schirmer, Jude M Przyborski, Katja Becker |
Journal | FEBS letters
(FEBS Lett)
Vol. 586
Issue 19
Pg. 3037-43
(Sep 21 2012)
ISSN: 1873-3468 [Electronic] England |
PMID | 22819813
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- DNA Primers
- DNA, Protozoan
- Protozoan Proteins
- Recombinant Fusion Proteins
- nucleoside triphosphate-adenylate kinase
- Adenylate Kinase
- adenylate kinase 1
- adenylate kinase 2
- Nucleoside-Phosphate Kinase
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Topics |
- Adenylate Kinase
(genetics, metabolism)
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Base Sequence
- Cytosol
(enzymology)
- DNA Primers
(genetics)
- DNA, Protozoan
(genetics)
- Humans
- Intracellular Membranes
(enzymology)
- Mitochondria
(enzymology)
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleoside-Phosphate Kinase
(genetics, metabolism)
- Plasmodium falciparum
(enzymology, genetics)
- Protozoan Proteins
(genetics, metabolism)
- Recombinant Fusion Proteins
(genetics, metabolism)
- Sequence Homology, Amino Acid
- Vacuoles
(enzymology)
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