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Subcellular localization of adenylate kinases in Plasmodium falciparum.

Abstract
Adenylate kinases (AK) play a key role in nucleotide signaling processes and energy metabolism by catalyzing the reversible conversion of ATP and AMP to 2 ADP. In the malaria parasite Plasmodium falciparum this reaction is mediated by AK1, AK2, and a GTP:AMP phosphotransferase (GAK). Here, we describe two additional adenylate kinase-like proteins: PfAKLP1, which is homologous to human AK6, and PfAKLP2. Using GFP-fusion proteins and life cell imaging, we demonstrate a cytosolic localization for PfAK1, PfAKLP1, and PfAKLP2, whereas PfGAK is located in the mitochondrion. PfAK2 is located at the parasitophorous vacuole membrane, and this localization is driven by N-myristoylation.
AuthorsJipeng Ma, Stefan Rahlfs, Esther Jortzik, R Heiner Schirmer, Jude M Przyborski, Katja Becker
JournalFEBS letters (FEBS Lett) Vol. 586 Issue 19 Pg. 3037-43 (Sep 21 2012) ISSN: 1873-3468 [Electronic] England
PMID22819813 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Chemical References
  • DNA Primers
  • DNA, Protozoan
  • Protozoan Proteins
  • Recombinant Fusion Proteins
  • nucleoside triphosphate-adenylate kinase
  • Adenylate Kinase
  • adenylate kinase 1
  • adenylate kinase 2
  • Nucleoside-Phosphate Kinase
Topics
  • Adenylate Kinase (genetics, metabolism)
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Base Sequence
  • Cytosol (enzymology)
  • DNA Primers (genetics)
  • DNA, Protozoan (genetics)
  • Humans
  • Intracellular Membranes (enzymology)
  • Mitochondria (enzymology)
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nucleoside-Phosphate Kinase (genetics, metabolism)
  • Plasmodium falciparum (enzymology, genetics)
  • Protozoan Proteins (genetics, metabolism)
  • Recombinant Fusion Proteins (genetics, metabolism)
  • Sequence Homology, Amino Acid
  • Vacuoles (enzymology)

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