Abstract | AIMS: The aim of this work was to investigate whether systemic oxidative imbalance that occurs in patients with systemic sclerosis affects red blood cell integrity. METHODS: RESULTS: A significant reduction (p<0.05) of intracellular total thiols and a significant loss (p<0.01) of glycophorin A, band 3 and CD47 was found in red blood cells from patients with limited cutaneous sclerosis. Conversely, a significant increase (p<0.01) of reactive oxygen species levels and CD47 expression was found in red blood cells from patients with diffuse cutaneous sclerosis. Phosphatidylserine externalization was significantly increased both in patients with limited and diffuse disease. Importantly, this increase was related with disease severity and nailfold capillaroscopy. CONCLUSIONS: Altogether these results suggest a reappraisal of the red blood cells as useful markers in the clinical management of the disease.
|
Authors | Antonello Giovannetti, Lucrezia Gambardella, Donatella Pietraforte, Edoardo Rosato, Anna Maria Giammarioli, Felice Salsano, Walter Malorni, Elisabetta Straface |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 30
Issue 2
Pg. 418-27
( 2012)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 22814239
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 S. Karger AG, Basel. |
Chemical References |
- Anion Exchange Protein 1, Erythrocyte
- CD47 Antigen
- Glycophorins
- Phosphatidylserines
- Reactive Oxygen Species
- Sulfhydryl Compounds
|
Topics |
- Adult
- Aged
- Anion Exchange Protein 1, Erythrocyte
(metabolism)
- CD47 Antigen
(metabolism)
- Cellular Senescence
(drug effects)
- Erythrocytes
(drug effects, metabolism)
- Female
- Flow Cytometry
- Glycophorins
(metabolism)
- Humans
- Male
- Microscopic Angioscopy
- Middle Aged
- Phosphatidylserines
(pharmacology)
- Pilot Projects
- Reactive Oxygen Species
(metabolism)
- Scleroderma, Systemic
(metabolism, physiopathology)
- Sulfhydryl Compounds
(metabolism)
|