Abstract | BACKGROUND: Wheat gluten comprises gliadins and glutenins. The high-molecular-weight (HMW) glutenin subunits (GS)-1Dy10 are toxic for patients with celiac disease (CD). This study aimed to assess whether CD patients mount a serological response to HMW-GS-1Dy10. METHODS: RESULTS: MALDI-TOF revealed a number of fragments matching known HMW-GS-1Dy10 sequences within both the deamidated and non-deamidated material. Evidence of deamidation of glutamine residues was found only within the human transglutaminase-treated material. Patients with untreated CD had significantly increased levels of serum antibodies to HMW-GS-1Dy10 compared to controls. Undeamidated HMW-GS-1Dy10 IgA antibodies had sensitivities and specificities of 72.5 and 78.26%, respectively. Deamidated HMW-GS-1Dy10 IgA antibodies had sensitivities and specificities of 76.8 and 65.2%. Undeamidated HMW-GS-1Dy10 IgG antibodies had sensitivities and specificities of 75.3 and 68.1%. Deamidated HMW-GS-1Dy10 IgG antibodies had sensitivities and specificities of 36.2 and 92.8%. CONCLUSION: Patients with untreated CD have raised antibody levels to HMW-GS-1Dy10, indicating the participation of these proteins in the adaptive immune response to gluten. Discrimination between CD patients and controls is not enhanced by deamidation of HMW-GS-1Dy10. Thus antibodies to these proteins are not useful markers for CD detection.
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Authors | H Julia Ellis, Pablo Lozano-Sanchez, Carmen Bermudo Redondo, Tanja Šuligoj, Federico Biagi, Paola I Bianchi, Gino R Corazza, Annalisa De Silvestri, Enzo Bravi, Ioanis Katakis, Ciara K O'Sullivan, Paul J Ciclitira |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 159
Issue 4
Pg. 428-34
( 2012)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 22813868
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 S. Karger AG, Basel. |
Chemical References |
- Antibodies
- Immunoglobulin A
- Protein Subunits
- Recombinant Proteins
- Glutens
- Gliadin
- Transglutaminases
- glutenin
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Topics |
- Adaptive Immunity
- Amino Acid Sequence
- Antibodies
(blood, immunology)
- Antibody Specificity
- Celiac Disease
(blood, diagnosis, immunology)
- Gliadin
(chemistry, immunology)
- Glutens
(chemistry, immunology)
- Humans
- Immunoglobulin A
(blood, immunology)
- Molecular Sequence Data
- Molecular Weight
- Protein Subunits
(chemistry, immunology)
- Recombinant Proteins
(chemistry, metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Transglutaminases
(metabolism)
- Triticum
(chemistry, immunology)
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