Beneficial effects of (-)-epigallocatechin gallate on ischemia-reperfusion testicular injury in rats.

Abstract

BACKGROUND/PURPOSE:

The purpose of the study was to determine the effect of (-)-epigallocatechin gallate (EGCG) on testicular ischemia-reperfusion injury in rats.

METHODS:

Forty male Wistar rats were assigned to 5 groups. A sham operation was performed on the animals in group 1. In group 2, after 4 hours of unilateral testicular ischemia, 4 hours of testicular reperfusion was performed with EGCG administered 1 hour before reperfusion. In group 3, the same surgical procedure as in group 2 was performed, but without EGCG. Serum superoxide dismutase activity, creatine kinase, and lactate dehydrogenase were then measured in blood samples from groups 1 to 3. In group 4, after 4 hours of unilateral testicular ischemia, testicular reperfusion was performed. In group 5, the same procedure as in group 4 was performed, but with EGCG administered 1 hour before reperfusion. For groups 4 and 5, bilateral orchiectomy was performed for histologic examination 4 weeks after reperfusion was started.

RESULTS:

Serum superoxide dismutase activity was significantly higher in group 2 than in group 3. The ratios of bilateral testicular weight, mean seminiferous tubule diameter, and germinal epithelial cell thickness were significantly higher in group 5 than in group 4.

CONCLUSIONS:

Therapy with EGCG before reperfusion might exert protective effects via antioxidant activities in a rat experimental model of testicular ischemia-reperfusion injury.

• Authors: Akihide Sugiyama, Masahiro Chiba, Tomokazu Nakagami, Shinya Kawano, Yutaka Sanada, Takuma Tajiri, Akira Toki
• Journal: Journal of pediatric surgery (J Pediatr Surg) Vol. 47 Issue 7 Pg. 1427-32 (Jul 2012) ISSN: 1531-5037 [Electronic] United States
• PMID: 22813808 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Biomarkers
• Catechin
• epigallocatechin gallate

Topics

• Animals
• Antioxidants (therapeutic use)
• Biomarkers (blood)
• Catechin (analogs & derivatives, therapeutic use)
• Disease Models, Animal
• Male
• Postoperative Complications (blood, pathology, prevention & control)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (blood, etiology, pathology, prevention & control)
• Spermatic Cord Torsion (surgery)