Multi-compartmental vaccine delivery system for enhanced immune response to gp100 peptide antigen in melanoma immunotherapy.

Abstract

PURPOSE:

To develop a multi-compartmental vaccine delivery system for safe and efficient delivery of the gp100 peptide antigen in melanoma immunotherapy.

METHODS:

Water-in-oil-in-water (W/O/W) multiple emulsion-based multi-compartmental vaccine delivery system containing the gp100 peptide was prepared by a two-step emulsification method. In vivo prophylactic and active immunization effectiveness of the novel squalane oil-containing gp100 vaccine was evaluated in the murine B16 melanoma model and compared with that of an incomplete Freund's adjuvant (IFA)-based vaccine.

RESULTS:

Morphological evaluation of the W/O/W multiple emulsions showed that the oil-droplets were homogenously dispersed with the gp100 peptide encapsulated in an inner aqueous phase. Immunization with the gp100 peptide delivered in the W/O/W multiple emulsions-based vaccine resulted in increased protection against tumor challenge compared to IFA-based vaccine (p < 0.05, n = 8) signifying induction of enhanced anti-tumor immunity. In addition, serum Th1 cytokine levels and immuno-histochemistry of excised tumor tissues indicated activation and local infiltration of antigen specific cytotoxic T-lymphocytes into and/or surrounding the tumor mass. Moreover, the newly developed vaccine formulation did not induce any overt systemic toxicity.

CONCLUSION:

Novel W/O/W multiple emulsions-based vaccine efficiently delivers the gp100 peptide antigen to induce cell-mediated anti-tumor immunity and offers an alternate, safe vaccine delivery system.

• Authors: Mayurkumar Kalariya, Srinivas Ganta, Mansoor Amiji
• Journal: Pharmaceutical research (Pharm Res) Vol. 29 Issue 12 Pg. 3393-403 (Dec 2012) ISSN: 1573-904X [Electronic] United States
• PMID: 22806408 (Publication Type: Journal Article)

Chemical References

• Cancer Vaccines
• Cytokines
• Emulsions
• Oils
• gp100 Melanoma Antigen

Topics

• Animals
• Cancer Vaccines (administration & dosage, immunology, therapeutic use)
• Cytokines (blood, immunology)
• Drug Delivery Systems
• Emulsions (chemistry)
• Immunization
• Melanoma, Experimental (blood, immunology, prevention & control)
• Mice
• Mice, Inbred C57BL
• Oils (chemistry)
• T-Lymphocytes, Cytotoxic (immunology)
• gp100 Melanoma Antigen (administration & dosage, immunology, therapeutic use)