Comparative proteomics analysis of colorectal cancer.

Abstract

BACKGROUND AND OBJECTIVE:

Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins.

MATERIALS AND METHODS:

Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI- TOF mass spectra.

RESULTS:

Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, including HSPB1 and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings.

CONCLUSIONS:

Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

• Authors: Jun-Jiang Wang, Ying Liu, Yang Zheng, Feng Lin, Guan-Fu Cai, Xue-Qing Yao
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 13 Issue 4 Pg. 1663-6 ( 2012) ISSN: 2476-762X [Electronic] Thailand
• PMID: 22799385 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A4
• HSP27 Heat-Shock Proteins
• HSPB1 protein, human
• Heat-Shock Proteins
• Molecular Chaperones
• Proteins

Topics

• Annexin A4 (metabolism)
• Colon (metabolism)
• Colorectal Neoplasms (metabolism)
• Down-Regulation
• Electrophoresis, Gel, Two-Dimensional
• HSP27 Heat-Shock Proteins (metabolism)
• Heat-Shock Proteins
• Humans
• Intestinal Mucosa (metabolism)
• Molecular Chaperones
• Proteins (metabolism)
• Proteomics
• Rectum (metabolism)
• Up-Regulation