Abstract |
The purpose of this study is to evaluate in vivo efficacy and loco-regional distribution of a doxorubicin (DOX)-loaded Polyoxyl 35 Castor Oil ( Cremophor EL, CrEL) noncovalent modified single-walled carbon nanotubes (SWNTs) formulation in a sarcoma tumor model after intratumoral injection. The drug loaded SWNTs were successfully prepared via physical absorption, which was confirmed by UV-vis-NIR absorbance spectra and dynamic light scattering assay. Solid tumor models were obtained by injecting mouse sarcoma 180 cells into the thighs of ICR mice. CrEL-SWNTs-DOX, CrEL-SWNTs, free DOX and saline (control) were intratumorally injected after 5 days post transplantation. The biodistribution studies demonstrated that intratumoral delivery of CrEL-SWNTs-DOX resulted in longer drug retention time in tumor, higher tumor level (27.6-fold than that of free DOX), as well as less accumulation in other solid tissues, especially in heart. Furthermore, in vivo anti- tumor activity results showed that CrEL-SWNTs-DOX could effectively suppress the tumor growth than free DOX and the control, attributing to its enhanced intratumoral DOX level. The histopathological findings revealed that the new carbon nanomaterials were a safe vehicle for topical drug delivery systems. It is concluded that this noncovalent modification of carbon nanotubes by CrEL for anticancer agents might be a promising alternative for cancer treatment.
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Authors | Hongzhuo Liu, Hui Xu, Yan Wang, Zhonggui He, Sanming Li |
Journal | Drug development and industrial pharmacy
(Drug Dev Ind Pharm)
Vol. 38
Issue 9
Pg. 1031-8
(Sep 2012)
ISSN: 1520-5762 [Electronic] England |
PMID | 22794192
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- Nanotubes, Carbon
- Pharmaceutical Vehicles
- cremophor EL
- Doxorubicin
- Glycerol
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Topics |
- Absorption
- Animals
- Antibiotics, Antineoplastic
(administration & dosage, metabolism, pharmacokinetics, therapeutic use)
- Cell Line, Tumor
- Doxorubicin
(administration & dosage, metabolism, pharmacokinetics, therapeutic use)
- Drug Compounding
- Drug Delivery Systems
- Female
- Glycerol
(adverse effects, analogs & derivatives, chemistry)
- Hydrophobic and Hydrophilic Interactions
- Injections, Intralesional
- Mice
- Mice, Inbred ICR
- Nanotubes, Carbon
(adverse effects, chemistry)
- Neoplasm Transplantation
- Pharmaceutical Vehicles
(adverse effects, chemistry)
- Sarcoma 180
(drug therapy, metabolism, pathology)
- Solubility
- Tissue Distribution
- Tumor Burden
(drug effects)
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