[Serum level of MMP-2 in early breast cancer and its correlation with circulating tumor cells].

Abstract

OBJECTIVE:

To explore the serum levels of MMP-2 in early breast cancer and its clinical significance and correlation with the circulating tumor cells in the patients.

METHODS:

The serum levels of MMP-2 in breast fibroadenoma (n = 10) and breast cancer (n = 72) were detected by enzyme-linked immunosorbent assay (ELISA). And CK19 mRNA was measured by quantitative reverse transcription-polymerase chain reaction in blood as an index of circulating tumor cells in the patients.

RESULTS:

The serum level of MMP-2 in breast cancer was significantly higher than that in breast fibroadenoma (12.24 vs 2.21 µg/L, P = 0.003). Subgroup analysis showed that the serum levels of MMP-2 were higher in HER-2 positive patients than those in HER-2 negative ones (16.77 vs 10.02 µg/L, P = 0.022). CK19 mRNA was found in blood samples from 30 cases of breast cancer and the positive rate was 41.7%. However, it was not detected in the patients with breast fibroadenoma. The mean levels of serum MMP-2 were (15.48 ± 2.02) and (7.16 ± 1.55) µg/L in CK19 positive and negative patients respectively. Significant difference existed between two groups (P = 0.013).

CONCLUSION:

The serum level of MMP-2 in early breast cancer is closely correlated with blood micrometastasis. And its high level may be an important contributing factor for the metastasis of breast cancer.

• Authors: Ju-liang Zhang, Qing Yao, Jiang-hao Chen, Ting Wang, Hui Wang, Jing Fan, Ling Wang
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 92 Issue 16 Pg. 1104-6 (Apr 24 2012) ISSN: 0376-2491 [Print] China
• PMID: 22781768 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Keratin-19
• MMP2 protein, human
• Matrix Metalloproteinase 2

Topics

• Adult
• Breast Neoplasms (blood, pathology)
• Female
• Humans
• Keratin-19 (blood)
• Matrix Metalloproteinase 2 (blood)
• Middle Aged
• Neoplasm Micrometastasis
• Neoplastic Cells, Circulating