[Expression of phosphorylated STAT5 in bone marrow hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria before and after in vitro G-CSF or SCF stimulation].

Abstract

OBJECTIVE:

To study the expressions of phosphorylated STAT5 (P-STAT5) in CD34(+)CD59(-) and CD34(+)CD59(+) bone marrow cells of the patients with paroxysmal nocturnal hemoglobinuria (PNH) before and after in vitro G-CSF or SCF stimulation, then evaluate the functions of G-CSF and SCF receptors in PNH clone cells.

METHODS:

Bone marrow mononuclear cells (BMMNC) of 26 PNH patients and 14 normal controls were stimulated in vitro with G-CSF (100 ng/ml) or SCF (100 ng/ml) for 10 min. Before and after these stimulations, the mean fluorescence intensity (MFI) of P-STAT5 in CD34(+)CD59(+) BMMNC and CD34(+)CD59(-) BMMNC were measured by flow cytometry.

RESULTS:

(1) The P-STAT5 MFI was (24 ± 18) in unstimulated CD34(+)CD59(-) cells. And it was significantly lower than that in unstimulated CD34(+)CD59(+) cells of PNH patients (64 ± 49) and normal controls (61 ± 33) (both P < 0.01). No statistic difference existed between the latter two. (2) The P-STAT5 MFI was (36 ± 35) in G-CSF stimulated CD34(+)CD59(-) cells of PNH patients. And it was significantly lower than that in G-CSF stimulated CD34(+)CD59(+) cells of PNH patients (124 ± 84) and normal controls (116 ± 59) (both P < 0.01). There was no statistic difference between the latter two. (3) The P-STAT5 MFI was (34 ± 27) in SCF stimulated CD34(+)CD59(-) cells of PNH patients. And it was significantly lower than that in SCF stimulated CD34(+)CD59(+) cells of PNH patients (124 ± 97) and normal controls (128 ± 62) (both P < 0.01). And no statistic difference existed between the latter two. (4) The increased P-STAT5 MFI in G-CSF stimulated CD34(+)CD59(-) cells of PNH patients was significantly lower than that in G-CSF stimulated CD34(+)CD59(+) cells of PNH patients and normal controls (both P < 0.01). No statistic difference existed between the latter two. The increase of P-STAT5 MFI in SCF stimulated CD34(+)CD59(-) cells of PNH patients was significantly lower than that in SCF stimulated CD34(+)CD59(+) cells of PNH patients and normal controls (both P < 0.01). No statistic difference existed between the latter two.

CONCLUSION:

There is a lower expression of P-STAT5 expressed in G-CSF or SCF stimulated PNH clone cells compared to that in normal clone cells.

• Authors: Shao-xue Ding, Rong Fu, Hong-lei Wang, Tian Zhang, Er-bao Ruan, Wen Qu, Yong Liang, Guo-jin Wang, Xiao-ming Wang, Hong Liu, Yu-hong Wu, Jia Song, Hua-quan Wang, Li-min Xing, Jing Guan, Jun Wang, Li-juan Li, Zong-hong Shao
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 92 Issue 14 Pg. 956-9 (Apr 10 2012) ISSN: 0376-2491 [Print] China
• PMID: 22781567 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• STAT5 Transcription Factor
• Granulocyte Colony-Stimulating Factor
• Proto-Oncogene Proteins c-kit

Topics

• Adolescent
• Adult
• Aged
• Bone Marrow (metabolism)
• Bone Marrow Cells (metabolism)
• Case-Control Studies
• Cells, Cultured
• Child
• Female
• Flow Cytometry
• Granulocyte Colony-Stimulating Factor (pharmacology)
• Hematopoietic Stem Cells (metabolism)
• Hemoglobinuria, Paroxysmal (etiology, metabolism)
• Humans
• Male
• Middle Aged
• Phosphorylation
• Proto-Oncogene Proteins c-kit (pharmacology)
• STAT5 Transcription Factor (metabolism)
• Young Adult