HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Multi-phase extraction of glycoraphanin from broccoli using aminium ionic liquid-based silica.

AbstractINTRODUCTION:
Glucosinolates, a class of phytochemicals found in broccoli, have attracted recent interest due to the potential health benefits associated with their dietary intake. Glucoraphanin, the most common glucosinolate in broccoli can be converted to a known cancer chemopreventive agent. Multi-phase extraction in solid-phase extraction cartridges was developed to simultaneously extract and separate this compound.
OBJECTIVE:
Multi-phase extraction with functionalised ionic liquid-based silica as a sorbent was used to simultaneously extract and separate glucoraphanin from broccoli.
METHODOLOGY:
The sorbent and broccoli sample were packed into a single cartridge, and a fixed volume of water was then used to extract and remove the target compound from the sample to the sorbent over 15 repetitions. The sorbent was then washed with n-hexane to remove any interference and the target compound was eluted with water-1% acetic acid (vol.).
RESULTS:
Under the optimised condition, 0.038 mg/g of glucoraphanin was obtained by multi-phase extraction with 0.2 g of sorbent.
CONCLUSION:
The adsorption isotherm allowed investigation of the interactions between the sorbent and target compound and provided evidence for the accuracy of this method. The low deviation error, small amount of solvents required, highly selective separation and stability of the method justify further research.
AuthorsMinglei Tian, Wentao Bi, Kyung Ho Row
JournalPhytochemical analysis : PCA (Phytochem Anal) 2013 Jan-Feb Vol. 24 Issue 1 Pg. 81-6 ISSN: 1099-1565 [Electronic] England
PMID22777845 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 John Wiley & Sons, Ltd.
Chemical References
  • Glucosinolates
  • Hexanes
  • Imidoesters
  • Ionic Liquids
  • Oximes
  • Sulfoxides
  • n-hexane
  • Silicon Dioxide
  • glucoraphanin
Topics
  • Adsorption
  • Brassica (chemistry)
  • Chemical Fractionation (instrumentation, methods)
  • Glucosinolates (isolation & purification)
  • Hexanes (chemistry)
  • Imidoesters (isolation & purification)
  • Ionic Liquids (chemistry)
  • Oximes
  • Reproducibility of Results
  • Silicon Dioxide (chemistry)
  • Sulfoxides

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: