Flavonol-containing phosphorylated pullulan may attenuate pulp inflammation.

Abstract

AIM:

To find possible reagents to minimize inflammatory responses by using an established pulpitis models for the purpose of developing new pulp-capping materials, and to test the possible use of phosphorylated pullulan as a carrier for such an anti-inflammatory reagent.

METHODOLOGY:

Co-culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells. The effects of two flavonoids, luteolin and quercetin, as anti-inflammatory reagents, and phosphorylated pullulan, which potentially achieves a sufficient marginal sealing to hydroxyapatite and slowly releases luteolin, as a carrier for flavonoids, were tested.

RESULTS:

Flavonols, particularly luteolin, dramatically attenuated inflammatory cytokine production, which was augmented by co-cultures. Luteolin was successfully enclosed by phosphorylated pullulan. Finally, it was confirmed that luteolin released from phosphorylated pullulan was effective in reducing cytokine production by co-cultures.

CONCLUSIONS:

Combination of phosphorylated pullulan and luteolin could be potentially used in the treatment of dental pulp inflammation.

• Authors: J Yonehiro, Y Yoshida, A Yamashita, S Yoshizawa, K Ohta, N Kamata, T Okihara, F Nishimura
• Journal: International endodontic journal (Int Endod J) Vol. 46 Issue 2 Pg. 119-27 (Feb 2013) ISSN: 1365-2591 [Electronic] England
• PMID: 22775284 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 International Endodontic Journal.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• CCL2 protein, human
• Chemokine CCL2
• Chemokine CCL5
• Drug Carriers
• Drug Combinations
• Flavonoids
• Glucans
• Interleukin-6
• Interleukin-8
• Pulp Capping and Pulpectomy Agents
• Tumor Necrosis Factor-alpha
• pullulan
• Quercetin
• Luteolin

Topics

• Anti-Inflammatory Agents, Non-Steroidal (pharmacology, therapeutic use)
• Cell Line, Transformed
• Cells, Cultured
• Chemokine CCL2 (biosynthesis)
• Chemokine CCL5 (biosynthesis)
• Coculture Techniques
• Dental Pulp (cytology, drug effects, metabolism)
• Drug Carriers (chemistry, pharmacology, therapeutic use)
• Drug Combinations
• Flavonoids (chemistry, therapeutic use)
• Glucans (chemistry, pharmacology, therapeutic use)
• Interleukin-6 (biosynthesis)
• Interleukin-8 (biosynthesis)
• Luteolin (pharmacology, therapeutic use)
• Materials Testing
• Pulp Capping and Pulpectomy Agents (therapeutic use)
• Pulpitis (drug therapy)
• Quercetin (pharmacology, therapeutic use)
• Tumor Necrosis Factor-alpha (biosynthesis)