Increased production of IL-5 and dominant Th2-type response in airways of Churg-Strauss syndrome patients.

Abstract

OBJECTIVE:

Churg-Strauss syndrome (CSS) is a rare systemic vasculitis associated with eosinophilia and asthma. We assessed the local immune response in airways of CSS patients with different activity of the disease.

METHODS:

Concentration of IL-5, CCL17, CCL22 and CCL26 (ELISA) together with cell expression of T-helper-related genes (real-time PCR array) were measured in bronchoalveolar lavage fluid (BALF) sampled from 11 patients with active CSS, 11 patients with CSS in remission and 9 control subjects with bronchial asthma.

RESULTS:

In active CSS, both BALF and blood eosinophil counts were increased (P<0.01). BALF cells in active disease were characterized by an increased expression of Th2 and regulatory-type transcripts: STAT6, STAT3, GATA3, IL4, IL5 and IL10 as compared with asthmatics, and STAT5A, CCR4, FOXP3, IL4, IL5 and IL10 when compared with inactive CSS. There was significant increase in BALF concentration of IL-5 and CCL26 in exacerbation of CSS. CCR4-active chemokines were detected more frequently in active disease. We found a strong positive correlation between clinical parameters of disease activity (BVAS, eosinophilia) and expression of IL4, IL5, IL10 and STAT5A.

CONCLUSION:

These results indicate that as compared with asthma, active-CSS patients have much stronger local Th2 response in the airways. Airway cells may contribute to lung eosinophilia in CSS by producing IL-5 and eosinophil active chemokines.

• Authors: Bogdan Jakiela, Wojciech Szczeklik, Hanna Plutecka, Barbara Sokolowska, Lucyna Mastalerz, Marek Sanak, Stanislawa Bazan-Socha, Andrzej Szczeklik, Jacek Musial
• Journal: Rheumatology (Oxford, England) (Rheumatology (Oxford)) Vol. 51 Issue 10 Pg. 1887-93 (Oct 2012) ISSN: 1462-0332 [Electronic] England
• PMID: 22772323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCL26 protein, human
• Chemokine CCL17
• Chemokine CCL22
• Chemokine CCL26
• Chemokines, CC
• Interleukin-5

Topics

• Adult
• Bronchoalveolar Lavage Fluid (chemistry, immunology)
• Chemokine CCL17 (analysis)
• Chemokine CCL22 (analysis)
• Chemokine CCL26
• Chemokines, CC (analysis)
• Churg-Strauss Syndrome (immunology, metabolism)
• Eosinophils (immunology)
• Female
• Gene Expression (immunology)
• Humans
• Interleukin-5 (analysis)
• Male
• Middle Aged