Effects of infliximab on bacterial translocation in experimental acute necrotizing pancreatitis.

Abstract

BACKGROUND & OBJECTIVES:

Translocation of bacteria from the gut is an important factor in the development of septic complications and mortality in acute pancreatitis (AP). The present study was designed to assess the effects of infliximab treatment on bacterial translocation (BT) in experimental acute necrotizing pancreatitis.

METHODS:

Male Sprague-Dawley rats (n=45) were allocated into three groups. AP was induced in group II (positive control, n=15) and group III (Infliximab; n=15) by retrograde injection of taurocholate into the common biliopancreatic duct. Group I rats (Sham; n=15) received normal saline infusion into the common biliopancreatic duct as placebo. Groups I and II were treated by normal saline and group III was treated with infliximab intraperitoneally on 6, 30 and 54 h after induction of pancreatitis. All surviving animals were killed 60 h after the induction of pancreatitis, and specimens were collected for amylase measurement as well as histopathologic and microbiologic examinations.

RESULTS:

Oedema, acinar cell necrosis, inflammatory infiltration, haemorrhage, fat necrosis and perivascular inflammation in group III rats were decreased with infliximab treatment when compared with group II (P<0.001). BT to mesentery lymph node in groups I, II and III were 20, 100 and 46 per cent, respectively. BT to peritoneum and pancreas in group III was lower than group II (P<0.05).

INTERPRETATION & CONCLUSIONS:

Infliximab administration resulted in beneficial effects on BT and histopathologic changes in the experimental necrotizing pancreatitis. Whether anti-TNF therapy has a role in prevention of complications of ANP needs to be established.

• Authors: Sezai Aydin, A Turan Isik, Bulent Unal, Bilgin Comert, Mustafa Ozyurt, Salih Deveci, Gokhan Ozgur, Omer Cengiz, Ilker Tasci, M Refik Mas
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 135 Issue 5 Pg. 656-61 (May 2012) ISSN: 0971-5916 [Print] India
• PMID: 22771595 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Taurocholic Acid
• Infliximab
• Amylases

Topics

• Acinar Cells (pathology)
• Amylases (blood)
• Animals
• Antibodies, Monoclonal (administration & dosage, adverse effects)
• Bacteria (classification, isolation & purification)
• Bacterial Translocation (drug effects)
• Humans
• Infliximab
• Male
• Models, Animal
• Pancreatic Ducts (microbiology)
• Pancreatitis, Acute Necrotizing (chemically induced, microbiology, pathology)
• Rats
• Rats, Sprague-Dawley
• Taurocholic Acid (pharmacology)