Triphala (PADMA) extract alleviates bronchial hyperreactivity in a mouse model through liver and spleen immune modulation and increased anti-oxidative effects.

Abstract

OBJECTIVES:

Triphala (TRP), a herbal extract from Tibetan medicine, has been shown to affect lymphocytes and natural killer T (NKT) cell function. We hypothesize that TRP could ameliorate bronchial hyperreactivity through immune-cell modulations.

METHODS:

Asthma mouse models were generated through intraperitoneal (IP) injections of ovalbumin (OVA)/2 weeks followed by repeated intranasal OVA challenges. Mice were then treated with normal saline (OVA/NS) or Triphala (OVA/TRP). Data were compared with mice treated with inhaled budesonide. All groups were assessed for allergen-induced hyperreactivity; lymphocytes from lungs, livers and spleens were analyzed for OVA-induced proliferation and their alterations were determined by flow cytometry. Oxidative reactivity using chemiluminescence, serum anti-OVA antibodies level and lung histology were assessed.

RESULTS:

Both TRP and budesonide significantly ameliorated functional and histological OVA-induced bronchial hyperreactivity. TRP had no effect on serum anti-OVA antibodies as compared with decreased levels following budesonide treatment. Furthermore, a significant increase in lung and spleen CD4 counts and a decrease in the liver were noted after TRP treatments. Bronchoalveolar fluid from TRP-treated animals but not from the budesonide-treated animals showed anti-oxidative effects.

CONCLUSION:

TRP and budesonide caused a significant decrease in bronchial reactivity. TRP treatment altered immune-cell distributions and showed anti-oxidative properties. These findings suggest that immune-cell modulation with TRP can ameliorate lung injury.

• Authors: Amjad Horani, David Shoseyov, Isaac Ginsburg, Rufayda Mruwat, Sarit Doron, Johnny Amer, Rifaat Safadi
• Journal: Therapeutic advances in respiratory disease (Ther Adv Respir Dis) Vol. 6 Issue 4 Pg. 199-210 (Aug 2012) ISSN: 1753-4666 [Electronic] England
• PMID: 22763675 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Asthmatic Agents
• Antioxidants
• Biomarkers
• Plant Extracts
• triphala
• Budesonide
• Ovalbumin
• Alanine Transaminase

Topics

• Administration, Inhalation
• Alanine Transaminase (blood)
• Animals
• Anti-Asthmatic Agents (administration & dosage, pharmacology)
• Antioxidants (administration & dosage, pharmacology)
• Asthma (drug therapy, immunology, metabolism, physiopathology)
• Biomarkers (blood)
• Bronchial Hyperreactivity (drug therapy, immunology, metabolism, physiopathology)
• Bronchial Provocation Tests
• Bronchoalveolar Lavage Fluid (chemistry)
• Budesonide (administration & dosage)
• CD4-Positive T-Lymphocytes (drug effects, immunology)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Disease Models, Animal
• Flow Cytometry
• Immunity, Humoral (drug effects)
• Liver (drug effects, immunology)
• Lung (drug effects, immunology, metabolism, physiopathology)
• Male
• Mice
• Mice, Inbred BALB C
• Ovalbumin
• Oxidative Stress (drug effects)
• Plant Extracts (administration & dosage, pharmacology)
• Spleen (drug effects, immunology)