HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The mediator complex subunit PFT1 interferes with COP1 and HY5 in the regulation of Arabidopsis light signaling.

Abstract
Arabidopsis (Arabidopsis thaliana) mutants hypersensitive to far-red light were isolated under a light program of alternating red and far-red light pulses and were named eid (for empfindlicher im dunkelroten Licht). The dominant eid3 mutant carries a missense mutation in a conserved domain of PHYTOCHROME AND FLOWERING TIME1 (PFT1), an important component of the plant mediator coactivator complex, which links promoter-bound transcriptional regulators to RNA polymerase II complexes. Epistatic analyses were performed to obtain information about the coaction between the mutated PFT1(eid3) and positively and negatively acting components of light signaling cascades. The data presented here provide clear evidence that the mutation mainly enhances light sensitivity downstream of phytochrome A (phyA) and modulates phyB function. Our results demonstrate that the Mediator component cooperates with CONSTITUTIVE PHOTORMORPHOGENIC1 in the regulation of light responses and that the hypersensitive phenotype strictly depends on the presence of the ELONGATED HYPOCOTYL5 transcription factor, an important positive regulator of light-dependent gene expression. Expression profile analyses revealed that PFT1(eid3) alters the transcript accumulation of light-regulated genes even in darkness. Our data further indicate that PFT1 regulates the floral transition downstream of phyA. The PFT1 missense mutation seems to create a constitutively active transcription factor by mimicking an early step in light signaling.
AuthorsCornelia Klose, Claudia Büche, Aurora Piñas Fernandez, Eberhard Schäfer, Eva Zwick, Thomas Kretsch
JournalPlant physiology (Plant Physiol) Vol. 160 Issue 1 Pg. 289-307 (Sep 2012) ISSN: 1532-2548 [Electronic] United States
PMID22760208 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anthocyanins
  • Arabidopsis Proteins
  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • HY5 protein, Arabidopsis
  • Nuclear Proteins
  • PFT1 protein, Arabidopsis
  • PHYA protein, Arabidopsis
  • PHYB protein, Arabidopsis
  • Phytochrome A
  • Phytochrome B
  • AT2G32950 protein, Arabidopsis
  • Ubiquitin-Protein Ligases
Topics
  • Anthocyanins (metabolism)
  • Arabidopsis (genetics, growth & development, metabolism, radiation effects)
  • Arabidopsis Proteins (genetics, metabolism)
  • Basic-Leucine Zipper Transcription Factors (genetics, metabolism)
  • Chromosome Mapping
  • Conserved Sequence
  • DNA-Binding Proteins
  • Epistasis, Genetic
  • Flowers (growth & development)
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Light
  • Mutation, Missense
  • Nuclear Proteins (genetics, metabolism)
  • Phenotype
  • Photoperiod
  • Phytochrome A (genetics, metabolism)
  • Phytochrome B (genetics, metabolism)
  • Protein Structure, Tertiary
  • Proteolysis
  • Signal Transduction
  • Transcription, Genetic
  • Ubiquitin-Protein Ligases (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: