Abstract |
The folate receptor (FR) is over-expressed on many solid tumors and has been exploited for targeted delivery of folic acid linked liposomes to cancer cells in vitro. In the present study, we developed a novel folic acid (FA) conjugated chitosan (CTS) grafted thiazole orange (TO) complex (FA-CTS-TO), and the formation can be used to label tumor cells. The structure of TO derivatives was confirmed by (1)H NMR and MS, and the fluorescence probe of FA-CTS-TO complex was confirmed by Fourier transform infrared analysis and Differential thermal analysis. The in vitro and in vivo of FA-CTS-TO complex were tested in breast cancer cells and the results showed a high targeting specificity in tumor cells with FR over-expressed. Such prominent fluorescence properties demonstrate again that FA-CTS-TO complex as a tumor targeting fluorescence probe is appropriate for breast cancer cells.
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Authors | Xue-Ning Fei, Yin Liu, Chao Li |
Journal | Journal of fluorescence
(J Fluoresc)
Vol. 22
Issue 6
Pg. 1555-61
(Nov 2012)
ISSN: 1573-4994 [Electronic] Netherlands |
PMID | 22752402
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amines
- Benzothiazoles
- Fluorescent Dyes
- Folate Receptors, GPI-Anchored
- Quinolines
- thiazole orange
- Serum Albumin, Bovine
- Chitosan
- Folic Acid
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Topics |
- Amines
(chemistry)
- Animals
- Benzothiazoles
(chemistry)
- Breast Neoplasms
(diagnosis, pathology)
- Cattle
- Chitosan
(chemistry)
- Drug Design
- Early Detection of Cancer
- Fluorescent Dyes
(chemical synthesis, chemistry, metabolism)
- Folate Receptors, GPI-Anchored
(metabolism)
- Folic Acid
(chemistry)
- Humans
- MCF-7 Cells
- Quinolines
(chemistry)
- Serum Albumin, Bovine
(metabolism)
- Temperature
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