Type A
neurotoxin (NTX) of Clostridium botulinum was purified by a simple procedure using a
lactose gel column. The toxicity of this purified toxin preparation was retained for at least 1 year at -30°C by supplementation with either 0.1%
albumin or 0.05%
albumin plus 1%
trehalose. When purified NTX was used to treat 49 patients with
urinary incontinence caused by either refractory idiopathic or neurogenic detrusor overactivity, 36 patients showed significant improvement in symptoms. These beneficial effects were also observed in cases of
prostatic hyperplasia. The results obtained with NTX were similar to that of
Botox. The effects of NTX on
trigeminal neuralgia induced by infraorbital nerve constriction (IoNC) in rats were also studied. Trigeminal ganglion neurons from ipsilateral to IoNC exhibited significantly faster onset of
FM4-64 release than
sham-operated contralateral neurons.
Intradermal injection of NTX in the area of IoNC alleviated IoNC-induced
pain behavior and reduced the exaggerated
FM4-64 release in trigeminal ganglion neurons.