Cytotoxic ellagitannins from Reaumuria vermiculata.

Abstract

Three ellagitannins and one disulfated flavonol were isolated from the aerial parts of Reaumuria vermiculata L. Besides that, 16 known compounds were characterized as well. The structures of all compounds were elucidated on the basis of spectroscopic data including 1D and 2D NMR and ESI HR-FTMS. The in vivo antioxidant activity using the oxygen radical absorbance capacity (ORAC) method, of the extract, its column fractions and two of the isolated ellagitannins was accomplished. In addition, a possible cytotoxicity of the extract and two of the new ellagitannins on HaCaT human keratinocytes and the activity of both compounds against the prostate cancer cell line (PC-3) were also assessed, whereby a potent cytotoxicity with IC₅₀ less than 1 μg/ml was determined for both compounds. Besides, the extract exhibited a potential cytotoxic effect against four different solid tumor cell lines, namely liver (Huh-7), colorectal (HCT-116), breast (MCF-7) and prostate (PC-3). The IC₅₀s were found to be substantially low (ranged from 1.3±0.15 to 2.4±0.22 μg/ml) with relatively low resistance possibility reaching to 0% in the case of Huh-7 cell.

• Authors: Mahmoud A Nawwar, Nahla A Ayoub, Mohamed A El-Rai, Fatma Bassyouny, Eman S Mostafa, Ahmed M Al-Abd, Manuela Harms, Kristian Wende, Ulrike Lindequist, Michael W Linscheid
• Journal: Fitoterapia (Fitoterapia) Vol. 83 Issue 7 Pg. 1256-66 (Oct 2012) ISSN: 1873-6971 [Electronic] Netherlands
• PMID: 22742959 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Antioxidants
• Hydrolyzable Tannins
• Plant Extracts

Topics

• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology, therapeutic use)
• Antioxidants (isolation & purification, pharmacology, therapeutic use)
• Carcinoma (drug therapy)
• Cell Line, Tumor
• Female
• Humans
• Hydrolyzable Tannins (isolation & purification, pharmacology, therapeutic use)
• Keratinocytes (drug effects)
• Male
• Molecular Structure
• Neoplasms (drug therapy)
• Phytotherapy
• Plant Components, Aerial
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Tamaricaceae (chemistry)