[Protective effects and mechanisms of OSR on primary cultured hippocampus neurons subjected to anoxic injury in neonatal rat].

Abstract

OBJECTIVE:

To investigate the protective effects of oxysophoridine (OSR) on primary cultured hippocampus neurons subjected to anoxia injury in neonatal rats and its mechanism.

METHOD:

The model of anoxia injury of hippocampus neurons in neonatal rats were primarily cultured in vitro by physical oxygen deficiency using glucose-free culture fluid. The survival rate of neurons, the leaking rate of lactate dehydrogenase (LDH), the intracellular contents of malondialdehyde (MDA) and nitric oxide (NO), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and nitric oxide synthase (NOS) were measured. The intracellular free calcium concentration ([Ca2+]i) in hippocampus neurons were detected with Ca(2+)-sensitive dual wavelength fluorescence spectrophotometer.

RESULT:

Neuron death occurred in the anoxia injury model group with increase of LDH leaking rate, the contents of NO, MDA, intracellular [Ca2+] and the elevated activity of NOS while decreased activities of SOD and GSH-PX. The hippocampus neurons subjected to anoxia injury were alleviated in OSR (0.625, 5, 10 microg x L(-1)) group.

CONCLUSION:

OSR has significant protective effects on hippocampus neurons subjected to anoxic injury. The mechanism of its protective effect may relate to its reduction of calcium overload and against oxidation injury.

• Authors: Jing Zhao, Yang Wu, Miao Sun, Jie Wang, Yunhong Li, Kuai Zhang, Jianqiang Yu
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 37 Issue 1 Pg. 94-8 (Jan 2012) ISSN: 1001-5302 [Print] China
• PMID: 22741470 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Drugs, Chinese Herbal
• Protective Agents
• oxysophoridine
• Malondialdehyde
• Glutathione Peroxidase
• Nitric Oxide Synthase
• Superoxide Dismutase

Topics

• Alkaloids (administration & dosage)
• Animals
• Cells, Cultured
• Drugs, Chinese Herbal (administration & dosage)
• Female
• Glutathione Peroxidase (metabolism)
• Hippocampus (cytology, drug effects, enzymology, metabolism)
• Humans
• Hypoxia (drug therapy, enzymology, metabolism, prevention & control)
• Malondialdehyde (metabolism)
• Neurons (cytology, drug effects, enzymology, metabolism)
• Nitric Oxide Synthase (metabolism)
• Protective Agents (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Sophora (chemistry)
• Superoxide Dismutase (metabolism)