Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae.

Abstract

TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.

• Authors: Annette Søndergaard, Alvaro San Millan, Alfonso Santos-Lopez, Signe M Nielsen, Bruno Gonzalez-Zorn, Niels Nørskov-Lauritsen
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 56 Issue 9 Pg. 4958-60 (Sep 2012) ISSN: 1098-6596 [Electronic] United States
• PMID: 22733069 (Publication Type: Journal Article)

Chemical References

• Recombinases
• beta-Lactams
• Ampicillin
• Tn3 resolvase
• Transposon Resolvases
• RNA-Dependent RNA Polymerase
• beta-Lactamases
• beta-lactamase TEM-1

Topics

• Ampicillin (pharmacology)
• Base Sequence
• Haemophilus influenzae (genetics, isolation & purification)
• Microbial Sensitivity Tests
• Molecular Sequence Data
• Open Reading Frames
• Plasmids
• RNA-Dependent RNA Polymerase (genetics)
• Recombinases (genetics)
• Transformation, Bacterial
• Transposon Resolvases (genetics)
• beta-Lactam Resistance (genetics)
• beta-Lactamases (genetics)
• beta-Lactams (pharmacology)