Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.

Abstract

BACKGROUND:

Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known.

OBJECTIVE:

To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients.

METHODS:

Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 μg/mL) by flow cytometry and cytokine production.

RESULTS:

Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls.

CONCLUSION:

Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.

• Authors: Enrique Gómez, Araceli Díaz-Perales, Leticia Tordesillas, Inmaculada Doña, María J Torres, Ana B Blázquez, Francisca Gómez, Miguel Blanca, Cristobalina Mayorga
• Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 109 Issue 1 Pg. 52-8 (Jul 2012) ISSN: 1534-4436 [Electronic] United States
• PMID: 22727158 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Chemical References

• Allergens
• Antigens, CD
• Antigens, Plant
• B7-1 Antigen
• B7-2 Antigen
• CXCL8 protein, human
• Fluoresceins
• Immunoglobulins
• Interleukin-8
• Membrane Glycoproteins
• Plant Proteins
• Pru p 3 allergen
• 6-carboxyfluorescein diacetate

Topics

• Adolescent
• Adult
• Allergens (immunology, metabolism)
• Antigens, CD (immunology, metabolism)
• Antigens, Plant (immunology)
• B7-1 Antigen (immunology, metabolism)
• B7-2 Antigen (immunology, metabolism)
• Case-Control Studies
• Cell Proliferation
• Dendritic Cells (immunology, metabolism)
• Female
• Flow Cytometry
• Fluoresceins
• Food Hypersensitivity (immunology, metabolism)
• Humans
• Immunoglobulins (immunology, metabolism)
• Interleukin-8 (immunology, metabolism)
• Lymphocyte Activation (immunology)
• Male
• Membrane Glycoproteins (immunology, metabolism)
• Middle Aged
• Monocytes (immunology, metabolism)
• Plant Proteins
• Prunus (immunology)
• Th1 Cells (immunology, metabolism)
• Th1-Th2 Balance
• Th2 Cells (immunology, metabolism)
• CD83 Antigen