Abstract | BACKGROUND AND AIM:
Histamine plays important physiological roles in upper gastrointestinal tract and acts via the H2 receptor. A polymorphism -1018 G>A (rs2067474) was identified in an enhancer element of the HRH2 promoter. We attempted to clarify the associations of this polymorphism with the progression of gastric mucosal atrophy. METHODS: Gastric mucosa samples were obtained from 398 subjects with no malignancies. The rs2067474 genotype was determined by PCR-SSCP method. The degree of gastritis was assessed in 366 subjects and serum pepsinogen (PG) I/II levels were measured in 108 subjects. The subjects with atrophy score higher or equal to 2 and metaplasia score higher or equal to1 were classified into the severe atrophic gastritis group (SA group). RESULTS: The -1018G>A minor allele frequencies in SA and non-SA groups were 8.02% and 13.3%, respectively (p=0.057). The -1018 GG homozygote had a significantly high risk for gastric mucosal atrophy (OR: 2.03, 95%CI: 1.03-4.01, p=0.042). In H. pylori positive subjects, GG homozygote was a more significant risk factor for gastric mucosal atrophy (OR: 2.32, 95%CI: 1.12-4.81, p=0.023). In addition, in the subjects older than 60 years, GG homozygote had also a significant risk for gastric mucosal atrophy (OR: 2.63, 95%CI: 1.15-6.00, p=0.022). In -1018 GG homozygote, PG I/II ratio was significantly lower in H. pylori positive than negative subjects and was significantly decreased with age (p=0.0032 by ANOVA), whereas it was not in the A carrier. CONCLUSIONS: Our results suggest that HRH2 -1018 GG homozygote is a risk factor for the severity of gastric mucosal atrophy under the influence of H. pylori infection, especially in older subjects.
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Authors | Hideto Yamada, Tomomitsu Tahara, Hisakazu Shiroeda, Ranji Hayashi, Takashi Saito, Masakatsu Nakamura, Mutsumi Tsuchishima, Mikihiro Tsutsumi, Tomoyuki Shibata, Tomiyasu Arisawa |
Journal | Journal of gastrointestinal and liver diseases : JGLD
(J Gastrointestin Liver Dis)
Vol. 21
Issue 2
Pg. 139-43
(Jun 2012)
ISSN: 1842-1121 [Electronic] Romania |
PMID | 22720301
(Publication Type: Journal Article)
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Chemical References |
- Receptors, Histamine H2
- Pepsinogen A
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Topics |
- Adult
- Age Factors
- Aged
- Female
- Gastric Mucosa
(pathology)
- Gastritis, Atrophic
(enzymology, genetics, microbiology)
- Gene Frequency
- Genetic Predisposition to Disease
- Helicobacter Infections
(complications, enzymology)
- Helicobacter pylori
- Heterozygote
- Homozygote
- Humans
- Male
- Middle Aged
- Pepsinogen A
(blood)
- Polymorphism, Genetic
- Polymorphism, Single-Stranded Conformational
- Receptors, Histamine H2
(genetics)
- Severity of Illness Index
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