Abstract | BACKGROUND: METHOD: To measure the inner diameter of the vessels, a rapid whole-body freezing method was used. RESULTS: Injection of the selective TP agonists U46619 and I-BOP constricted the fetal DA at embryonic day 19 (e19) and e21 in a dose-dependent manner. Of note, U46619 also exerted a vasoconstrictive effect on two different types of postnatal PDA models: premature PDA and hypoxia-induced PDA. We also found that U46619 constricted the ex vivo DA ring to a greater extent than it constricted the ex vivo aorta. Furthermore, we found that U46619 at lower concentrations (up to 0.05 mg/g of body weight) had a minimal vasoconstrictive effect on other vessels and did not induce microthrombosis in the pulmonary capillary arteries. CONCLUSION: Low-dose TP stimulation constricts the DA with minimal adverse effects at least in rat neonates and our results could point to an alternative potent vasoconstrictor for PDA.
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Authors | Tomohiro Yokota, Takashi Aida, Yasuhiro Ichikawa, Takayuki Fujita, Utako Yokoyama, Susumu Minamisawa |
Journal | Pediatric research
(Pediatr Res)
Vol. 72
Issue 2
Pg. 129-36
(Aug 2012)
ISSN: 1530-0447 [Electronic] United States |
PMID | 22717688
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bridged Bicyclo Compounds, Heterocyclic
- Fatty Acids, Unsaturated
- Receptors, Thromboxane A2, Prostaglandin H2
- 7-(3-(3-hydroxy-4-(4'-iodophenoxy)-1-butenyl)-7-oxabicyclo(2.2.1)heptan-2-yl)-5-heptenoic acid
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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Topics |
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
(pharmacology)
- Analysis of Variance
- Animals
- Blotting, Western
- Bridged Bicyclo Compounds, Heterocyclic
(pharmacology)
- Capillaries
(pathology)
- Cryopreservation
- Dose-Response Relationship, Drug
- Ductus Arteriosus
(drug effects, growth & development)
- Ductus Arteriosus, Patent
(prevention & control)
- Fatty Acids, Unsaturated
(pharmacology)
- Fetus
- Pulmonary Alveoli
(blood supply)
- Rats
- Rats, Wistar
- Real-Time Polymerase Chain Reaction
- Receptors, Thromboxane A2, Prostaglandin H2
(agonists)
- Vasoconstriction
(drug effects)
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