Abstract |
The overexpression of mitochondrial transcription factor A (TFAM) attenuates the decrease in mtDNA copy number after myocardial infarction, ameliorates pathological hypertrophy, and markedly improves survival. However, non-transgenic strategy to increase mtDNA for the treatment of pathological hypertrophy remains unknown. We produced recombinant human TFAM protein (rhTFAM). rhTFAM rapidly entered into mitochondria of cultured cardiac myocytes. rhTFAM increased mtDNA and abolished the activation of nuclear factor of activated T cells (NFAT), which is well known to activate pathological hypertrophy. rhTFAM attenuated subsequent morphological hypertrophy of myocytes as well. rhTFAM would be an attractive molecule in attenuating cardiac pathological hypertrophy.
|
Authors | Takeo Fujino, Tomomi Ide, Masayoshi Yoshida, Ken Onitsuka, Atsushi Tanaka, Yuko Hata, Motohiro Nishida, Takako Takehara, Takaaki Kanemaru, Naoyuki Kitajima, Shinya Takazaki, Hitoshi Kurose, Dongchon Kang, Kenji Sunagawa |
Journal | Mitochondrion
(Mitochondrion)
Vol. 12
Issue 4
Pg. 449-58
(Jul 2012)
ISSN: 1872-8278 [Electronic] Netherlands |
PMID | 22709542
(Publication Type: Journal Article)
|
Copyright | Copyright © 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved. |
Chemical References |
- DNA-Binding Proteins
- Mitochondrial Proteins
- NFATC Transcription Factors
- Recombinant Proteins
- Transcription Factors
- mitochondrial transcription factor A
|
Topics |
- Animals
- Cells, Cultured
- DNA-Binding Proteins
(genetics, metabolism)
- Humans
- Hypertrophy
(physiopathology)
- Male
- Mice
- Mitochondrial Proteins
(genetics, metabolism)
- Myocytes, Cardiac
(physiology)
- NFATC Transcription Factors
(antagonists & inhibitors)
- Recombinant Proteins
(genetics, metabolism)
- Signal Transduction
- Transcription Factors
(genetics, metabolism)
|