Abstract | CONTEXT: OBJECTIVE: DESIGN AND SETTING: We conducted a prospective study at the University Hospitals of the Leicester National Health Service Trust. PARTICIPANTS AND METHODS: Fallopian tubes collected from women with ectopic pregnancy and nonpregnant women with regular menstrual cycles were used for quantification of endocannabinoids by ultra-HPLC tandem mass spectrometry, were fixed in formalin for immunohistochemistry, and had RNA extracted for RT-quantitative PCR or protein extracted for immunoblotting. RESULTS: CONCLUSION: High anandamide levels and reduced expression of CB1 and FAAH may play a role in ectopic implantation.
|
Authors | Alpha K Gebeh, Jonathon M Willets, Emma L Marczylo, Anthony H Taylor, Justin C Konje |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 97
Issue 8
Pg. 2827-35
(Aug 2012)
ISSN: 1945-7197 [Electronic] United States |
PMID | 22701012
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Arachidonic Acids
- Endocannabinoids
- Polyunsaturated Alkamides
- RNA, Messenger
- Receptor, Cannabinoid, CB1
- Receptor, Cannabinoid, CB2
- Amidohydrolases
- fatty-acid amide hydrolase
- anandamide
|
Topics |
- Adult
- Amidohydrolases
(analysis, genetics, physiology)
- Arachidonic Acids
(analysis)
- Endocannabinoids
- Fallopian Tubes
(chemistry, metabolism)
- Female
- Humans
- Immunohistochemistry
- Middle Aged
- Polyunsaturated Alkamides
(analysis)
- Pregnancy
- Pregnancy, Ectopic
(metabolism)
- RNA, Messenger
(analysis)
- Receptor, Cannabinoid, CB1
(analysis, genetics, physiology)
- Receptor, Cannabinoid, CB2
(physiology)
|