Abstract |
Although many proteins have been described involved in Escherichia coli colonization and infection, only few reports have shown lectins as important components in these processes. Because the mechanisms underlying E. coli colonization process involving lectins are not fully understood, we sought to identify the presence of other non-described lectins in E. coli. Here, we isolated a 75-kDa protein from E. coli on Sepharose column and identified it as ferric aerobactin receptor (IutA). Since IutA is controversially associated with virulence of some E. coli strains, mainly in uropathogenic E. coli (UPEC), we evaluated the presence of iutA gene in UPEC isolated from patients with urinary infection. This gene was present in only 38% of the isolates, suggesting a weak association with virulence. Because there is a redundancy in the siderophore-mediated uptake systems, we suggest that IutA can be advantageous but not essential for UPEC.
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Authors | Taise Natali Landgraf, Alan Berlese, Fabricio Freitas Fernandes, Mariani Lima Milanezi, Roberto Martinez, Ademilson Panunto-Castelo |
Journal | Revista latino-americana de enfermagem
(Rev Lat Am Enfermagem)
2012 Mar-Apr
Vol. 20
Issue 2
Pg. 340-5
ISSN: 1518-8345 [Electronic] Brazil |
PMID | 22699735
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Outer Membrane Proteins
- aerobactin receptor
- Sepharose
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Topics |
- Bacterial Outer Membrane Proteins
(isolation & purification, physiology)
- Bacteriological Techniques
(methods)
- Escherichia coli Infections
(microbiology)
- Humans
- Sepharose
- Uropathogenic Escherichia coli
(pathogenicity)
- Virulence
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