Neoplastic cell response to tiopronin-coated gold nanoparticles.

Abstract

The present study characterized the in vitro biological response of a comprehensive set of cancer cell lines to gold nanoparticles (2.7 nm) coated with tiopronin (AuNPs-TP). Our findings suggest that upon entering cells, the AuNPs-TP are sequestered in vacuoles such as endosomes and lysosomes, and mostly localize in perinuclear areas. Peak cell accumulation was achieved at 8 hours after incubation. L929 and H520 cells showed more than 75% surviving fraction when treated with 0.5 mg/mL of AuNPs-TP for 24 hours, whereas the surviving fractions were 60% in MCF-7 and 20% in HeLa cells. Reactive oxygen species (ROS) production by the AuNPs-TP was dependent on cell line and exposure time. Antioxidants inhibited ROS generation to various extents, with glutathione and tiopronin being most effective. Overall, exposure time, concentration of the AuNPs-TP, and cell line influenced neoplastic cell response. Furthermore, the mechanism of cytotoxicity of the AuNPs-TP was found to be ROS generation.

FROM THE CLINICAL EDITOR:

This study describes the basic intracellular characteristics of Tiopronin-Au nanoparticles from the standpoint of their anti-cancer activity in different cancer cell cultures.

• Authors: Lei Cui, Payam Zahedi, Justin Saraceno, Robert Bristow, David Jaffray, Christine Allen
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 9 Issue 2 Pg. 264-73 (Feb 2013) ISSN: 1549-9642 [Electronic] United States
• PMID: 22687897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Reactive Oxygen Species
• Gold
• Tiopronin
• Glutathione

Topics

• Animals
• Antioxidants (metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Glutathione (metabolism)
• Gold (chemistry, pharmacokinetics, pharmacology)
• Humans
• Mice
• Nanoparticles (chemistry)
• Neoplasms (drug therapy, metabolism)
• Reactive Oxygen Species (metabolism)
• Tiopronin (chemistry, pharmacokinetics, pharmacology)