Abstract |
Cu(II) complexes with 1,10-orthophenanthroline (phen) show cytotoxic and antitumoral effects. To enhance and exploit these features, we studied complexes containing one or two phen units together with N,N'-substituted-imidazolidine-2-thione (L). We synthesized and structurally characterized the precursor molecule Cu(phen)( OH(2))(2)(OClO(3))(2), and determined the complex formation constants of [Cu(phen)(L)](2+). We studied the cytotoxic activity of [ Cu(phen)(2)(L)](ClO(4))(2) versus human hematologic (CCRF-CEM and CCRF-SB) and solid tumor-derived cell lines (K-MES-1, DU-145). The cytotoxic activities, in the 1-3 μM range, show that our Cu(II)-complexes possess comparable inhibitory activities against both leukemia and carcinoma cells, unlike the majority of antineoplastic agents, usually more potent against hematologic cancer cells than against solid tumor cells. Because the free Cu(II) ion is reduced by glutathione (GSH), we studied the reactivity of our complexes with GSH, providing evidence that no redox reaction occurred under the chosen experimental conditions. Complex formation equilibria were present, studied by spectrophotometric titrations. The redox properties of the prepared compounds were also investigated by cyclic voltammetry, confirming that the mixed Cu(II) complexes were resistant to reduction.
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Authors | Tiziana Pivetta, Francesco Isaia, Gaetano Verani, Carla Cannas, Laura Serra, Carlo Castellano, Francesco Demartin, Federica Pilla, Matteo Manca, Alessandra Pani |
Journal | Journal of inorganic biochemistry
(J Inorg Biochem)
Vol. 114
Pg. 28-37
(Sep 2012)
ISSN: 1873-3344 [Electronic] United States |
PMID | 22687563
(Publication Type: Journal Article)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Coordination Complexes
- Imidazolidines
- Phenanthrolines
- Thiones
- Ethylenethiourea
- Copper
- Glutathione
- 1,10-phenanthroline
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Topics |
- Antineoplastic Agents
(chemical synthesis, pharmacology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Coordination Complexes
(chemical synthesis, pharmacology)
- Copper
(chemistry)
- Crystallography, X-Ray
- Drug Screening Assays, Antitumor
- Ethylenethiourea
- Glutathione
(chemistry)
- Humans
- Imidazolidines
(chemistry)
- Kinetics
- Molecular Structure
- Oxidation-Reduction
- Phenanthrolines
(chemistry)
- Thiones
(chemistry)
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