Anticancer effects of residual powder from Barley-Shochu distillation remnants against the orthotopic xenograft mouse models of hepatocellular carcinoma in vivo.

Abstract	Barely-Shochu is a traditional Japanese liquor distilled from fermented barley with Saccharomyces cerevisiae. Barely-Shochu distillation remnants (SDR) are by-products in the manufacturing process of barley-Shochu. We have already reported on valuable powder from Shochu distillation remnants (PSDR) including antioxidative compounds such as polyphenols. In this study, we investigated the therapeutic effects of barely-PSDR against orthotopic xenograft mouse models of hepatocellular carcinoma (HCC) in vivo. We constructed a mouse model of HCC by orthotopical inoculation of HepG2 cells into the liver of SCID mice. Barely-PSDR (2250 mg/kg) was orally treated once each day for 21 d after the inoculation of HepG2 cells. The livers were removed from anaesthetized mice after the treatment with barely-PSDR and fixed in formalin. The liver sections were analyzed by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) methods. Remarkably high reduction of tumorigenesis was obtained in the mouse models of HCC after the oral administration of barely-PSDR in vivo. Induction of apoptosis in the liver section on the mouse models treated with barely-PSDR was observed. Furthermore, prolonged survival was obtained. Thus, therapeutic effects of barely-PSDR without side effects on the orthotopic xenograft mouse models were revealed for the first time.
Authors	Masahiro Ohgidani, Hideaki Ichihara, Koichi Goto, Yoko Matsumoto, Ryuichi Ueoka
Journal	Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 35 Issue 6 Pg. 984-7 ( 2012) ISSN: 1347-5215 [Electronic] Japan
PMID	22687544 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents, Phytogenic Plant Extracts
Topics	Animals Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use) Apoptosis (drug effects) Carcinoma, Hepatocellular (drug therapy, pathology) Distillation Female Hep G2 Cells Hordeum Humans Liver Neoplasms (drug therapy, pathology) Mice Mice, SCID Plant Extracts (pharmacology, therapeutic use) Tumor Burden (drug effects) Xenograft Model Antitumor Assays

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