Abstract | PURPOSE:
Sphingosine kinase (SphK) 1 is an oncogenic enzyme promoting transformation, proliferation, and survival of a number of human tumor cells. However, its effect on colon cancer cell behavior has not been fully clarified. METHODS: RESULTS: Overexpression of SphK1 after plasmid transfection markedly enhanced LOVO cell viability and invasiveness and reduced cell apoptosis. In contrast, inhibition of SphK1 by DMS and shRNA significantly suppressed cell viability and invasiveness but promoted cell apoptosis. SphK1 increased the constitutive expression of extracellular signal-regulated kinase1/2 (ERK1/2) but reduced the constitutive expression of p38 mitogen-activated protein kinase (MAPK). Blocking ERK1/2 pathway inhibited the biological effects induced by overexpression of SphK1. Blocking p38 MAPK pathway reversed the effects of DMS and SphK1 shRNA. Moreover, SphK1 was required for the production of MMP-2/9 and uPA in tumor cells, which was suppressed by ERK1/2 inhibitor U0126, but enhanced by the p38 MAPK inhibitor SB203580. CONCLUSIONS: SphK1 enhances colon cancer cell proliferation and invasiveness, meanwhile suppressing cell apoptosis. SphK1 promoting the secretion of MMP-2/9 and uPA via activation of ERK1/2 and suppression of p38 MAPK pathways maybe the molecular mechanisms for its regulation of the malignant behavior of colon cancer cell.
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Authors | Shi-Quan Liu, Jie-An Huang, Meng-Bin Qin, Ying-Jie Su, Ming-Yu Lai, Hai-Xing Jiang, Guo-Du Tang |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 27
Issue 12
Pg. 1569-78
(Dec 2012)
ISSN: 1432-1262 [Electronic] Germany |
PMID | 22684547
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Protein Kinase Inhibitors
- RNA, Messenger
- RNA, Small Interfering
- Phosphotransferases (Alcohol Group Acceptor)
- sphingosine kinase
- Extracellular Signal-Regulated MAP Kinases
- Urokinase-Type Plasminogen Activator
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
- N,N-dimethylsphingosine
- Sphingosine
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Topics |
- Apoptosis
(drug effects, genetics)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Colonic Neoplasms
(enzymology, genetics, pathology)
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- MAP Kinase Signaling System
(drug effects)
- Matrix Metalloproteinase 2
(biosynthesis, metabolism)
- Matrix Metalloproteinase 9
(biosynthesis, metabolism)
- Neoplasm Invasiveness
- Phosphotransferases (Alcohol Group Acceptor)
(antagonists & inhibitors, genetics, metabolism)
- Protein Kinase Inhibitors
(pharmacology)
- RNA, Messenger
(genetics, metabolism)
- RNA, Small Interfering
(metabolism)
- Sphingosine
(analogs & derivatives, pharmacology)
- Up-Regulation
(genetics)
- Urokinase-Type Plasminogen Activator
(metabolism)
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