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The neuroprotective effect of eupatilin against ischemia/reperfusion-induced delayed neuronal damage in mice.

Abstract
Eupatilin, a pharmacologically active flavone derived from the Artemisia plant species, has been reported to have anti-oxidant, anti-inflammatory, anti-allergic, and anti-tumor activities. In the present study, we investigated whether eupatilin exhibits neuroprotective activities against ischemia/reperfusion-induced delayed neuronal injury in mice. Transient global cerebral ischemia was induced in mice by bilateral common carotid artery occlusion (BCCAO) for 15 min followed by reperfusion for 4 days. Eupatilin (1, 3, or 10 mg/kg, p.o.) was administered immediately after the reperfusion. Histochemical studies showed that eupatilin (10 mg/kg) increased the number of viable cells detected by Nissl staining and decreased the number of degenerating neuronal cells detected by Fluoro-Jade B staining in the hippocampal CA1 region. Western blotting indicated that eupatilin further increased the level of Akt phosphorylation at 8h after BCCAO. Furthermore, wortmannin, a phosphatidylinositol 3-kinase inhibitor, attenuated the eupatilin-induced increase of Akt phosphorylation. In addition, wortmannin completely reversed the eupatilin-induced neuroprotective effects observed at 4 days after reperfusion. These findings suggest that eupatilin is a promising therapeutic agent against global cerebral ischemia-induced neuronal damage and that its neuroprotective effects may be mediated in part by increased Akt phosphorylation.
AuthorsMudan Cai, Phuong-Thuy T Phan, Jin Gyu Hong, Dong Hyun Kim, Jong Min Kim, Se Jin Park, Xiaotong Liu, Jeong Eun Han, Haeil Park, Ji Woong Choi, Jong Hoon Ryu
JournalEuropean journal of pharmacology (Eur J Pharmacol) Vol. 689 Issue 1-3 Pg. 104-10 (Aug 15 2012) ISSN: 1879-0712 [Electronic] Netherlands
PMID22683875 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Drugs, Chinese Herbal
  • Flavonoids
  • Neuroprotective Agents
  • eupatilin
Topics
  • Animals
  • Brain Ischemia (pathology, prevention & control)
  • Drugs, Chinese Herbal (pharmacology, therapeutic use)
  • Flavonoids (pharmacology, therapeutic use)
  • Male
  • Mice
  • Neurons (drug effects, pathology)
  • Neuroprotective Agents (pharmacology, therapeutic use)
  • Reperfusion Injury (pathology, prevention & control)
  • Time Factors

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