The neuroprotective effect of eupatilin against ischemia/reperfusion-induced delayed neuronal damage in mice.

Eupatilin, a pharmacologically active flavone derived from the Artemisia plant species, has been reported to have anti-oxidant, anti-inflammatory, anti-allergic, and anti-tumor activities. In the present study, we investigated whether eupatilin exhibits neuroprotective activities against ischemia/reperfusion-induced delayed neuronal injury in mice. Transient global cerebral ischemia was induced in mice by bilateral common carotid artery occlusion (BCCAO) for 15 min followed by reperfusion for 4 days. Eupatilin (1, 3, or 10 mg/kg, p.o.) was administered immediately after the reperfusion. Histochemical studies showed that eupatilin (10 mg/kg) increased the number of viable cells detected by Nissl staining and decreased the number of degenerating neuronal cells detected by Fluoro-Jade B staining in the hippocampal CA1 region. Western blotting indicated that eupatilin further increased the level of Akt phosphorylation at 8h after BCCAO. Furthermore, wortmannin, a phosphatidylinositol 3-kinase inhibitor, attenuated the eupatilin-induced increase of Akt phosphorylation. In addition, wortmannin completely reversed the eupatilin-induced neuroprotective effects observed at 4 days after reperfusion. These findings suggest that eupatilin is a promising therapeutic agent against global cerebral ischemia-induced neuronal damage and that its neuroprotective effects may be mediated in part by increased Akt phosphorylation.
AuthorsMudan Cai, Phuong-Thuy T Phan, Jin Gyu Hong, Dong Hyun Kim, Jong Min Kim, Se Jin Park, Xiaotong Liu, Jeong Eun Han, Haeil Park, Ji Woong Choi, Jong Hoon Ryu
JournalEuropean journal of pharmacology (Eur J Pharmacol) Vol. 689 Issue 1-3 Pg. 104-10 (Aug 15 2012) ISSN: 1879-0712 [Electronic] Netherlands
PMID22683875 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Drugs, Chinese Herbal
  • Flavonoids
  • Neuroprotective Agents
  • eupatilin
  • Animals
  • Brain Ischemia (pathology, prevention & control)
  • Drugs, Chinese Herbal (pharmacology, therapeutic use)
  • Flavonoids (pharmacology, therapeutic use)
  • Male
  • Mice
  • Neurons (drug effects, pathology)
  • Neuroprotective Agents (pharmacology, therapeutic use)
  • Reperfusion Injury (pathology, prevention & control)
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: