Abstract | AIMS:
Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the inflammatory process and immune response. The aim of the study was to investigate ICAM-1 expression in the kidneys of spontaneously hypertensive rats (SHRs) and the relationship between the level of ICAM-1 and renal damage. METHODS: Male Wistar-Kyoto (WKY) rat and SHR models were employed. Blood pressure (BP) was recorded using tail cuff method. Twenty-four hour proteinuria and β(2)-microglobulin (β(2)-MG) were measured using biuret method and radioimmunity kits, respectively. Biochemical parameters were measured after the animals were killed. ICAM-1 expression in renal tissue was assessed using western blot and real-time polymerase chain reaction (PCR). RESULTS: It was observed that BP, proteinuria, and urine β(2)-MG were more increased in SHR groups than that in the same age WKY groups. In SHR groups, BP, proteinuria, and urine β(2)-MG at the 56th week were significantly higher than that at the 28th week. ICAM-1 protein and mRNA expression in SHR renal tissues was significantly increased in SHR groups compared with the same age in WKY rats. ICAM-l expression was positively correlated with proteinuria and urine β(2)-MG. CONCLUSION: This study demonstrated that the renal expression of ICAM-1 was increased in SHR with renal damage, and inflammation may be involved in the hypertensive renal damage.
|
Authors | Ding-Wu Zhang, Xin-Zhong Huang, Jian-Hua Wu, Ya-Pin Fan, Hui Shi |
Journal | Renal failure
(Ren Fail)
Vol. 34
Issue 7
Pg. 915-20
( 2012)
ISSN: 1525-6049 [Electronic] England |
PMID | 22681549
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- RNA, Messenger
- beta 2-Microglobulin
- Intercellular Adhesion Molecule-1
|
Topics |
- Animals
- Arterial Pressure
- Hypertension
(metabolism, pathology)
- Immunohistochemistry
- Intercellular Adhesion Molecule-1
(metabolism)
- Kidney
(pathology)
- Male
- Proteinuria
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Inbred SHR
- Rats, Inbred WKY
- beta 2-Microglobulin
(metabolism)
|