Electrically-responsive anti-adherent hydrogels for photodynamic antimicrobial chemotherapy.

Abstract

The loading of the photosensitisers meso-Tetra (N-methyl-4-pyridyl) porphine tetra tosylate (TMP), methylene blue (MB) and TMP with sodium dodecyl sulphate (SDS) into and release from hydrogels composed of the polyelectrolyte poly(methyl vinyl ether-co-maleic acid) crosslinked in a 2:1 ratio with PEG 10,000 were investigated as a potential rapid photodynamic antimicrobial chemotherapy (PACT) treatment for infected wounds using iontophoresis as a novel delivery method. Photosensitiser uptake was very high; (% TMP uptake; 95.53-96.72%) (% MB uptake; 90.58-93.26%) and was PMVE/MA concentration independent, whilst SDS severely limited TMP uptake (5.93-8.75%). Hydrogel hardness, compressibility and adhesiveness on the dermal surface of neonate porcine skin increased with PMVE/MA concentration and were significantly increased with SDS. The ionic conductivities of the hydrogels increased with PMVE/MA concentration. Drug release was PMVE/MA concentration independent, except for drug release under iontophoteric conditions for MB and TMP (without SDS). In just 15 min, the mean% drug concentrations released of TMP, TMP (with SDS) and MB using an electric current ranged from 22.30 to 64.72 μg ml(-1), 6.37-4.59 μg ml(-1) and 11.73-36.57 μg ml(-1) respectively. These concentrations were in excess of those required to induce complete kill of clinical strains of meticillin-resistant Staphylococcus aureus and Burkholderia cepacia. Thus these results support our contention that the iontophoteric delivery of TMP and MB using anti-adherent, electrically-responsive, PEG-crosslinked PMVE/MA hydrogels are a potential option in the rapid PACT treatment of infected wounds.

• Authors: Steven J Fallows, Martin J Garland, Corona M Cassidy, Michael M Tunney, Thakur Raghu Raj Singh, Ryan F Donnelly
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 114 Pg. 61-72 (Sep 03 2012) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 22677563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Infective Agents
• Hydrogels
• Maleates
• Photosensitizing Agents
• Polyethylenes
• Polyethylene Glycols
• poly(methyl vinyl ether-co-maleic anhydride)

Topics

• Anti-Infective Agents (chemical synthesis, chemistry, pharmacology)
• Burkholderia cepacia (drug effects)
• Electricity
• Hydrogels (chemistry)
• Light
• Maleates (chemistry)
• Methicillin-Resistant Staphylococcus aureus (drug effects)
• Photosensitizing Agents (chemical synthesis, chemistry, pharmacology)
• Polyethylene Glycols (chemistry)
• Polyethylenes (chemistry)